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Commensal bacteria protect against food allergen sensitization

ABSTRACT: Analysis of colonic epithelial cell gene expression in germ-free, Bacteroides uniformis-colonized, and Clostridia-colonized gnotobiotic mice. Bacteria were isolated from our SPF mouse facility and were used to selectively colonize germ-free mice. Germ free mice were left germ free or were colonized with Bacteroides uniformis or a consortium of Clostridia. Total RNA was extraced from colonic epithelial cells.

ORGANISM(S): Mus musculus

SUBMITTER: Cathryn Nagler

PROVIDER: E-GEOD-60039 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Commensal bacteria protect against food allergen sensitization

Project description:Analysis of colonic epithelial cell gene expression in germ-free, Bacteroides uniformis-colonized, and Clostridia-colonized gnotobiotic mice. Bacteria were isolated from our SPF mouse facility and were used to selectively colonize germ-free mice.

2014-08-15 | GSE60039 | GEO

Intestine-adapted bacterial symbiont response to polysaccharide rich and polysaccharide deficient diets in mice lacking fucosyltransferase-2

Project description:Analysis of Bacteroides thetaiotaomicron (BT) from the ceca of ex-germ free Fut2+ or Fut2- mice on polysaccharide rich or glucose rich polysaccharide deficient diet. BT is involved in the breakdown of plant polysaccharides and is also efficiently utilizes host glycans. BT-colonized mice represent a human gut ecosystem model. Results identify genes that may endow flexibility in adapting to dietary changes by mucosal foraging depending on host fucosylation status In vitro transcriptional profiles of Bacteroides thetaiotaomicron obtained from ceca of ex-germ free Fut2+ or Fut2- mice on polysaccharide rich or glucose rich polysaccharide deficient diet.

2014-04-21 | E-GEOD-45641 | biostudies-arrayexpress

Commensal yeast derived b-1,6-glucan induces regulatory T cells

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2021-04-21 | GSE126937 | GEO

To determine transcriptome of gnotobiotic mice fed fiber-rich and fiber-free diets

Project description:Despite accepted health benefits of dietary fiber, little is known about the mechanisms by which fiber deprivation impacts the gut microbiota and alters disease risk. Using a gnotobiotic model, in which mice were colonized with a synthetic human gut microbiota, we elucidated the functional interactions between dietary fiber, the gut microbiota and the colonic mucus barrier, which serves as a primary defence against pathogens. We show that during chronic or intermittent dietary fiber deficiency, the gut microbiota resorts to host-secreted mucus glycoproteins as a nutrient source, leading to erosion of the colonic mucus barrier. Dietary fiber deprivation promoted greater epithelial access and lethal colitis by the mucosal pathogen, Citrobacter rodentium, but only in the presence of a fiber-deprived microbiota that is pushed to degrade the mucus layer. Our work reveals intricate pathways linking diet, gut microbiome and intestinal barrier dysfunction, which could be exploited to improve health using dietary therapeutics. Germ-free mice (Swiss Webster) were colonized with synthetic human gut microbiota comprising of 14 species belonging to five different phyla (names of bacterial species: Bacteroides thetaiotaomicron, Bacteroides ovatus, Bacteroides caccae, Bacteroides uniformis, Barnesiella intestinihominis, Eubacterium rectale, Marvinbryantia formatexigens, Collinsella aerofaciens, Escherichia coli HS, Clostridium symbiosum, Desulfovibrio piger, Akkermansia muciniphila, Faecalibacterium prausnitzii and Roseburia intestinalis). These mice were fed either a fiber-rich diet or a fiber-free diet for about 6 weeks. The mice were then sacrificed and their cecal tissues were immediately flash frozen for RNA extraction. The extracted RNA was subjected to microarray analysis based on Mouse Gene ST 2.1 strips using the Affy Plus kit. Expression values for each gene were calculated using robust multi-array average (RMA) method.

2016-11-21 | GSE79067 | GEO

In vivo commensal control of Clostridioides difficile virulence

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2015-08-13 | E-GEOD-60751 | biostudies-arrayexpress

Commensal bacteria protect against food allergen sensitization.

Project description:Environmentally induced alterations in the commensal microbiota have been implicated in the increasing prevalence of food allergy. We show here that sensitization to a food allergen is increased in mice that have been treated with antibiotics or are devoid of a commensal microbiota. By selectively colonizing gnotobiotic mice, we demonstrate that the allergy-protective capacity is conferred by a Clostridia-containing microbiota. Microarray analysis of intestinal epithelial cells from gnotobiotic mice revealed a previously unidentified mechanism by which Clostridia regulate innate lymphoid cell function and intestinal epithelial permeability to protect against allergen sensitization. Our findings will inform the development of novel approaches to prevent or treat food allergy based on modulating the composition of the intestinal microbiota.

| S-EPMC4246970 | biostudies-literature

RNA-seq data of intestinal epithelial cells

Project description:We report that adhesion of microbes to intestinal epithelial cells is a critical cue for Th17 induction. SFB colonized in the intestine of mice can adhere to mouse small intestinal epithelial cells and induce intestinal Th17 cells. However, SFB colonized in rats cannot adhere to mouse intestinal epithelial cells and induce Th17 cells. Likewise, Citrobacter rodentium (WT) can adhere to mouse colonic epithelial cells and induce Th17 cells, but non-adherent mutant of C. rodentium (Δeae) cannot induce Th17 cells. To examine the influence of adherent bacteria on intestinal epithelial cells, we performed RNA seq. Germ free mice were orally inoculated with M-SFB or R-SFB and total RNA was isolated from small intestinal epithelial cells 1 week after inoculation. Alternatively, germ free mice were orally inoculated with C. rodentium WT or eae mutant and total RNA was isolated from colonic epithelial cells 5 days after inoculation. The gene expression of small intestinal epithelial cells isolated from small intestine of germ free mice (2 mice), mice monocolonized with M-SFB (2 mice) or R-SFB (3 mice), and colon of germ free mice (3 mice), mice monocolonized C. rodentium WT (3 mice) or eae mutant (3 mice).

2015-09-24 | E-GEOD-71734 | biostudies-arrayexpress

Open Array Custom Real-time quantitative PCR analysis of mouse colonic mucosa

Project description:Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays.

2019-10-01 | GSE121609 | GEO

Open Array Custom Real-time quantitative PCR analysis of mouse ileal mucosa

Project description:Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the ileal and colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays.

2019-10-01 | GSE121612 | GEO

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