Unknown,Transcriptomics,Genomics,Proteomics

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Effect of knock down of LASP-1 on basal-like breast cancer cells (MDA-MB-231)


ABSTRACT: Nuclear LASP-1 has a direct correlation with the overall survival of breast cancer patients. Gene expression analysis of MDA-MB-231S (sorted for high surface expression of CXCR4) and MDA-Bone-Un (Mouse bone metastasized MDA-MB-231 cells) human basal-like breast cancer cells cultured in 3D-Matrigel was performed. Changes in transcript levels of key microRNAs 29B1 and 29B2, miRLet7F1, miR519A1, MMP9, MMP1, FAM75D4, Interferons a7 and a17, Glycine receptor a3, CADM2 and claudin12 Non-silencing (control) and LASP-1 knock down MDA-MB-231-S and MDA-Bone-Un cells were cultured on 3D-Matrigel, total RNA was extracted and analyzed - 1 biological replicate each

ORGANISM(S): Homo sapiens

SUBMITTER: Dayanidhi Raman 

PROVIDER: E-GEOD-60323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex.

Duvall-Noelle N N   Karwandyar A A   Richmond A A   Raman D D  

Oncogene 20150518 9


Nuclear LASP-1 (LIM and SH3 protein-1) has a direct correlation with overall survival of breast cancer patients. In this study, immunohistochemical analysis of a human breast TMA showed that LASP-1 is absent in normal human breast epithelium but the expression increases with malignancy and is highly nuclear in aggressive breast cancer. We investigated whether the chemokines and growth factors present in the tumor microenvironment could trigger nuclear translocation of LASP-1.Treatment of human b  ...[more]

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