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Single-cell RNA-seq of mouse cerebral cortex

ABSTRACT: We have applied a recently developed, highly accurate and sensitive single-cell RNA-seq method (STRT/C1) to perform a molecular census of two regions of the mouse cerebral cortex: the somatosensory cortex and hippocampus CA1. We isolated cells fresh from somatosensory cortex (S1) and hippocampus CA1 area of juvenile (P22 - P32) CD1 mice, 33 males and 34 females. Cells were collected without selection, except that 116 cells were obtained by FACS from 5HT3a-BACEGFP transgenic mice. A total of 76 Fluidigm C1 runs were performed, each attempting 96 cell captures and resulting in 3005 high-quality single-cell cDNAs, containing Unique Molecular Identifiers allowing counting of individual mRNA molecules, even after PCR amplification.

ORGANISM(S): Mus musculus

SUBMITTER: Sten Linnarsson

PROVIDER: E-GEOD-60361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq.

Zeisel Amit A Muñoz-Manchado Ana B AB Codeluppi Simone S Lönnerberg Peter P La Manno Gioele G Juréus Anna A Marques Sueli S Munguba Hermany H He Liqun L Betsholtz Christer C Rolny Charlotte C Castelo-Branco Gonçalo G Hjerling-Leffler Jens J Linnarsson Sten S

Science (New York, N.Y.) 20150219 6226

The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified ...[more]

PMID: 25700174

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