Unknown,Transcriptomics,Genomics,Proteomics

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Identification of Adr1 regulated genes

ABSTRACT: Adr1 of Ustilago maydis is a protein kinase that is activated after separation from the regulatory subunit mediated by high cAMP levels. A copy of the gene under the control of the arabinose-inducible crg1 promotor was introduced into the Cbx locus of the wild type strain FB1 creating strain HE140. To monitor the transcriptional changes upon adr1 induction by arabinose, we conducted a time course experiment comparing the wild type transcriptional response before and 75 or 180 min after medium shift to complete medium containing arabinose, as well as the response of the mutant HE140 before and 75 or 180 min after induction of the additional copy of adr1 by arabinose. Keywords: gene induction Strains FB1 (control) and HE140 (a1b1 ipr[pcrg1:adr1]ips) were grown in CM medium containing glucose and were shifted to CM medium containing arabinose and grown for 75 or 180 min. Samples were taken before and after arabinose induction from two (glucose) or three (arabinose) biological replicates each.

ORGANISM(S): Ustilago maydis

SUBMITTER: Jan Schirawski 

PROVIDER: E-GEOD-6039 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A ferroxidation/permeation iron uptake system is required for virulence in Ustilago maydis.

Eichhorn Heiko H   Lessing Franziska F   Winterberg Britta B   Schirawski Jan J   Kämper Jörg J   Müller Philip P   Kahmann Regine R  

The Plant cell 20061130 11

In the smut fungus Ustilago maydis, a tightly regulated cAMP signaling cascade is necessary for pathogenic development. Transcriptome analysis using whole genome microarrays set up to identify putative target genes of the protein kinase A catalytic subunit Adr1 revealed nine genes with putative functions in two high-affinity iron uptake systems. These genes locate to three gene clusters on different chromosomes and include the previously identified complementing siderophore auxotroph genes sid1  ...[more]

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