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Global Regulation of Alternative Internal Exon Usage by mRNA 3' End Formation Factors [RNA-Seq]

ABSTRACT: RNA-seq analysis of samples from Non-silencing, RBFOX2 and CPSF2 knockdown cells We analyzed 6 samples using the Illumina HiSeq 2000. Sequence data was processed by DexSeq. Two biological replicates were performed for Non-silencing, RBFOX2 and CPSF2 knockdown cells

ORGANISM(S): Homo sapiens

SUBMITTER: Ashish Misra

PROVIDER: E-GEOD-60391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors.

Misra Ashish A Ou Jianhong J Zhu Lihua J LJ Green Michael R MR

Molecular cell 20150423 5

The mechanisms that regulate alternative precursor mRNA (pre-mRNA) splicing are largely unknown. Here, we perform an RNAi screen to identify factors required for alternative splicing regulation by RBFOX2, an RNA-binding protein that promotes either exon inclusion or exclusion. Unexpectedly, we find that two mRNA 3' end formation factors, cleavage and polyadenylation specificity factor (CPSF) and SYMPK, are RBFOX2 cofactors for both inclusion and exclusion of internal exons. RBFOX2 interacts with ...[more]

PMID: 25921069

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