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Genome-wide identification of BRAT- and PUM-associated mRNAs reveals PUM-independent functions for BRAT in translational repression and mRNA degradation during the Drosophila maternal-to-zygotic transition

ABSTRACT: Here, we analyze the RNA-binding preferences of the Drosophila melanogaster BRAT protein using RNAcompete. In the RNAcompete assay, purified GST-tagged BRAT is incubated with an excess of RNA pool and bound RNA from individual pulldown experiments are directly labeled and hybridized to a custom Agilent 244K microarray.

ORGANISM(S): synthetic construct

SUBMITTER: Debashish Ray

PROVIDER: E-GEOD-60498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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