Unknown,Transcriptomics,Genomics,Proteomics

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Copy number profile of human prostate cancer organoid lines using Agilent 1M aCGH platform

ABSTRACT: Advanced prostate cancer is a highly heterogenous disease with few in vitro models. We report generation of seven novel 3D organoid lines of patient-derived prostate cancer. We determine the copy number alterations of these lines using array CGH. They contain may classic alterations of prostate cancer such as lost of the short arm of chromosome 8 and gain of the long arm of chromosome 8. In addition, we found homozygous deleteion of tumor suppressor PTEN and CHD1 as well as the TMPRSS2-ERG interstitial deletion. Prostate cancer organoid lines, ~2 months after in vitro propagation, were used for profiling on Agilent 1M aCGH arrays per manufacturer's instructions. A pooled reference normal DNA was used as the reference.

ORGANISM(S): Homo sapiens

SUBMITTER: Yu Chen 

PROVIDER: E-GEOD-60612 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Organoid cultures derived from patients with advanced prostate cancer.

Gao Dong D   Vela Ian I   Sboner Andrea A   Iaquinta Phillip J PJ   Karthaus Wouter R WR   Gopalan Anuradha A   Dowling Catherine C   Wanjala Jackline N JN   Undvall Eva A EA   Arora Vivek K VK   Wongvipat John J   Kossai Myriam M   Ramazanoglu Sinan S   Barboza Luendreo P LP   Di Wei W   Cao Zhen Z   Zhang Qi Fan QF   Sirota Inna I   Ran Leili L   MacDonald Theresa Y TY   Beltran Himisha H   Mosquera Juan-Miguel JM   Touijer Karim A KA   Scardino Peter T PT   Laudone Vincent P VP   Curtis Kristen R KR   Rathkopf Dana E DE   Morris Michael J MJ   Danila Daniel C DC   Slovin Susan F SF   Solomon Stephen B SB   Eastham James A JA   Chi Ping P   Carver Brett B   Rubin Mark A MA   Scher Howard I HI   Clevers Hans H   Sawyers Charles L CL   Chen Yu Y  

Cell 20140904 1

The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells. The first seven fully characterized organoid lines recapitulate the molecular diversity of prostate cancer subtypes, including TMPRSS2-ERG fusion, SPOP mutation, SPINK1 overexpress  ...[more]

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