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DNA methylation analysis of the U937 cell line

ABSTRACT: DNA methylation profiling of gene promoters in the human myeloid cell line U937. Bisulphite converted DNA from wildtype U937 cells was hybridised to the Illumina Infinium HumanMethylation27 BeadChip to determine the methylation pattern of the U937 cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Carl Sanden

PROVIDER: E-GEOD-60734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The DEK oncoprotein binds to highly and ubiquitously expressed genes with a dual role in their transcriptional regulation.

Sandén Carl C Järvstråt Linnea L Lennartsson Andreas A Brattås Per Ludvik PL Nilsson Björn B Gullberg Urban U

Molecular cancer 20140912

<h4>Background</h4>The DEK gene is highly expressed in a wide range of cancer cells, and a recurrent translocation partner in acute myeloid leukemia. While DEK has been identified as one of the most abundant proteins in human chromatin, its function and binding properties are not fully understood.<h4>Methods</h4>We performed ChIP-seq analysis in the myeloid cell line U937 and coupled it with epigenetic and gene expression analysis to explore the genome-wide binding pattern of DEK and its role in ...[more]

PMID: 25216995

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