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Unknown,Transcriptomics,Genomics,Proteomics

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Characterization of FOXL2 and five nuclear receptors transcriptional function in mouse granulosa cells

ABSTRACT: FOXL2 is a lineage determining transcription factor in the ovary, but its direct targets and modes of action are not fully characterized. Interaction of FOXL2 with several members of the nuclear receptor familly of transcription factors has been described, and many nuclear receptors play a key role in ovarian biology. Here, we explore the targets of FOXL2 and five nuclear receptors in murine primary follicular cells. siRNA targeting FOXL2 or control siRNA were used in conjunction with siRNAs targeting five different nuclear receptors or control siRNAs (2x6=12 conditions). Each condition was analysed in completely independent biological duplicates (i.e. cell from different animals grown independently)

ORGANISM(S): Mus musculus

SUBMITTER: Adrien Georges

PROVIDER: E-GEOD-60764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FOXL2 and ESR1 binding sites in primary cells from mouse ovarian follicles

Project description:It has previously been shown that FOXL2 and ESR1 cooperate to repress the testis-determining gene Sox9 in murine granulosa cells, and suggested that FOXL2/ESR1 cooperation may be central to granulosa cell differentiation (Uhlenhaut et al., 2009). However, no study has so far compared the DNA-binding of FOXL2 and ESR1 at the genomic level or analyzed the impact of FOXL2 on ESR1 binding to its regulatory elements. Here, we have analyzed and compared the genomic locations recognized by ESR1 and FOXL2 in E2-treated primary murine granulosa cells. Input DNA, FOXL2 and ESR1 ChIP

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2014-10-29 | E-GEOD-60858 | biostudies-arrayexpress

Characterization of FOXL2 and five nuclear receptors transcriptional function in mouse granulosa cells

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2014-10-29 | GSE60764 | GEO

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2014-10-29 | GSE60770 | GEO

Mus musculus

Project description:Characterization of FOXL2 and five nuclear receptors transcriptional function in mouse granulosa cells

| PRJNA259529 | ENA

ChIP-chip by array of mouse ovaries from wild-type 5 week old Swiss mice using custom anit-Foxl2 polyclonal antibodies

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FOXL2 interaction with different binding partners regulates the dynamics of granulosa cell differentiation across ovarian development

Project description:FOXL2 is a transcription factor essential for female fertility, expressed in somatic cells of the ovary, notably granulosa cells. In the mouse, Foxl2 deletion leads to partial sex reversal postnatally. However, deletion of the gene in 8-week-old females leads to granulosa to Sertoli cell transdifferentiation. We hypothesised that different outcomes of Foxl2 deletion in embryonic versus adult ovary may depend on a different role played across ovarian development. Therefore, we characterised the dynamics of gene expression and chromatin accessibility changes in purified murine granulosa cells across key developmental stages (E14.5, 1 and 8 weeks). We then performed genome-wide identification of FOXL2 target genes and on-chromatin interacting partners by ChIP-SICAP. We found that FOXL2 regulates more genes at postnatal stages, through the interaction with factors regulating primordial follicle activation (PFA), such as NR5A2, and others regulating steroidogenesis including AR and ESR2. As a proof of principle experiment, we chose one FOXL2 interactor, Ubiquitin specific protease 7 (USP7) and showed that deletion of this gene in granulosa cells leads to a blockage of PFA, impaired ovary development and sterility. Our study constitutes a comprehensive resource for exploration of the molecular mechanisms of ovarian development and causes of female infertility.

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Transcription profiling of mouse KK1 granulosa cells transfected with Foxl2-VP16 and Foxl2-MAD

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Gene knockdown and overexpression of 402C>G FOXL2 in COV434 and KGN cells

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2012-11-05 | E-GEOD-39890 | biostudies-arrayexpress

Genome wide identification of FOXL2 binding sites in mouse fetal ovaries

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