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Expression data from +Pi and -Pi treeatment between WT and spx1spx2 double mutant

ABSTRACT: Fourteen days plants growth under hydroponic +P condition (200 µM) were treated with +P(200µM) or –P (no phosphate) for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying +Pi and -Pi condition between WT and spx1spx2 double mutant. Fourteen days plants growth under hydroponic +P condition (200 µM) were treated with +P(200µM) or –P (no phosphate) for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis.

ORGANISM(S): Oryza sativa

SUBMITTER: Chuanzao Mao

PROVIDER: E-GEOD-60823 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rice SPX1 and SPX2 inhibit phosphate starvation responses through interacting with PHR2 in a phosphate-dependent manner.

Wang Zhiye Z Ruan Wenyuan W Shi Jing J Zhang Li L Xiang Dan D Yang Chao C Li Changying C Wu Zhongchang Z Liu Yu Y Yu Yanan Y Shou Huixia H Mo Xiaorong X Mao Chuanzao C Wu Ping P

Proceedings of the National Academy of Sciences of the United States of America 20140930 41

In plants, sensing the levels of external and internal nutrients is essential for reprogramming the transcriptome and adapting to the fluctuating environment. Phosphate (Pi) is a key plant nutrient, and a large proportion of Pi starvation-responsive genes are under the control of Phosphate Starvation Response Regulator 1 (PHR1) in Arabidopsis (AtPHR1) and its homologs, such as Oryza sativa (Os)PHR2 in rice. AtPHR1 and OsPHR2 expression is not very responsive to Pi starvation, raising the questio ...[more]

PMID: 25271318

Similar Datasets

Expression data from +Pi and -Pi treeatment between WT and spx1spx2 double mutant

Project description:Fourteen days plants growth under hydroponic +P condition (200 µM) were treated with +P(200µM) or –P (no phosphate) for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying +Pi and -Pi condition between WT and spx1spx2 double mutant.

2014-10-03 | GSE60823 | GEO

Expression data from -Pi treatment between WT, phr1, phr3 and phr1phr2phr3 triple mutant

Project description:Fourteen days plants growth under hydroponic +P condition (200 μM) were treated under –P (no phosphate) condition for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis. We used microarrays to detail the global programme of gene expression underlying -Pi condition among WT and mutants of phr1, phr3 and triple mutant of phr1phr2phr3. Fourteen days plants growth under hydroponic +P condition (200 μM) were treated under –P (no phosphate) condition for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis.

2015-06-25 | E-GEOD-69010 | biostudies-arrayexpress

Expression data from -Pi treatment between WT, phr1, phr3 and phr1phr2phr3 triple mutant

2015-06-25 | GSE69010 | GEO

Transcriptomic study of the impact of iron toxicity on rice plant

Project description:transcriptomic study of the impact of iron toxicity on rice plant (Oryza sativa L.; cv M-bM-^@M-^XI Kong PaoM-bM-^@M-^Y ) after short term (3 days) or long term (3 weeks) exposure to ferrous iron (125 ppm). Twenty five days old rice seedlings were exposed to 0 or 125 mg/L ferrous iron for 3 days and 3 weeks in hydroponic culture. Comparison between control and iron stressed plants were done at the shoot and the root levels. The assays were replicated twice on two independent plant cultures. 8 samples, Two-condition experiment, control (0 ppm ferrous iron) vs. iron treated (125 ppm ferrous iron). Biological replicates: 2 replicates for comparison shoot 3 days of stress, root 3 days of stress, shoot 3 weeks of stress and root 3 weeks of stress.

2012-05-14 | E-GEOD-36299 | biostudies-arrayexpress

Expression data from Arabidopsis thaliana PHR1 primary targets genes under phosphate starvation stress

Project description:We performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) phr1 mutant plants expressing PHR1 in presence of cicloheximide, that inhibit protein translation, thus preventing any effect of PHR1 on the expression of indirect targets. Results show the primary target genes of PHR1 in the responses to Pi starvation. The analysis was performed in phr1 plants and phr1 plants overexpressing a fusion GR:PHR1 (OXGR:PHR1) whose activity is postranslationally controlled by dexamethasone (DEX), that allows studying gene expression upon PHR1 activation and the concomitant inhibition of translation with cycloheximide (CHX), thus preventing any effect of PHR1 on the expression of indirect targets. To perform this study, phr1 and OXGR:PHR1 phr1 plants were grown for seven days in complete Johnson liquid media (+Pi), 2 days in Pi-lacking media (-Pi) and then supplemented with 5 µM DEX and 10 µM CHX for 6 hours before harvesting. Three independent biological samples of total RNA from shoot and root were hybridized separately.

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An improved, low-cost, hydroponic system for growing Arabidopsis and other model plant species under sterility conditions (Arabidopsis transcriptional profile in early response to Pi starvation)

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Transcriptional response of durum wheat roots and leaves to continuous chromium stress

Project description:Chromium (Cr) is a highly toxic heavy metal for both mono and dicot plants, causing severe cellular damage and disturbances in plant growth, development, and reproduction. Its phytotoxicity results in serious changes in plant physiology, gene expression, and at a transgenerational level in genomic DNA methylation. Herein, transcriptional responses of durum wheat (Triticum turgidum L.) to continuous and basal Cr stress were explored in roots and leaves by RNAseq analysis. Plants cultivated in a hydroponic system supplemented with 2.5 and 10 µM Cr were compared with control plants through transcriptomic analysis after 50 days and for the biomass accumulation and seed yield after senescence.

2024-08-26 | E-MTAB-13952 | biostudies-arrayexpress

Transcriptome changes for Arabidopsis thaliana in response to lead exposure

Project description:Experiments were achieved on Arabidopsis thaliana. Transcriptional profiling of roots and shoots from plants treated with lead were compared to plants treated in similar conditions without lead. Four weeks old A. thaliana seedlings were treated in hydroponic cultures with Pb during 3 days, by adding or not 40 µM Pb(NO3)2.

2015-01-28 | GSE65333 | GEO

transcriptomic analysis of phosphatin effect-Phosphatin effect on Arabidopsis

Project description:cea11-02_phosphatin - transcriptomic analysis of phosphatin effect - Identification of transcriptomic modifications promoted by phosphatin (AC6) (a molecule mimicking Pi addition effects on Pi starved plants). - 40 µM Phosphatin was added to MS (diluted 10 times) containing 20µM phosphate medium and controls were grown on MS (diluted 10 times) containing 20µM phosphate or supplemented with 500 µM of Pi. For each ARN extraction their was 3 plates containing each 10 seedlings grown 13 days in vertical petri dishes.

2013-04-11 | GSE45937 | GEO

Molecular interaction between PHO2 and GIGANTEA reveals a new crosstalk between flowering time and Pi homeostasis in rice

Project description:Using a yeast to hybrid screen, it was found that PHO2, a key regulator of phosphate homeostasis, could interact with GIGANTEA, a key regulator of flowering time. In addition, mutant analysis revealed that both the pho2 and gi mutants shared similar phenotypes, both at the phosphate and flowering time levels. We used microarrays to analyse the effects of each mutation (pho2 or gi) on global gene expression, and assessed the proportion of genes similalry regulated in both mutants, in both shoots and roots. Wild-type (Nipponbare), pho2 and gi knock-out rice plants were grown to 21-days before harvesting root and shoot separately.

2013-07-01 | E-GEOD-46867 | biostudies-arrayexpress

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