Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

P63+/Krt5+ Distal Airway Stem Cells are Essential for Lung Regeneration (transplanted and damaged)


ABSTRACT: The possibility of lung regeneration has been long discounted due to the irreversible nature of chronic lung diseases. However, patients who sustain massive loss of lung tissue during acute infections often recover full pulmonary function. Correspondingly, we previously demonstrated lung regeneration in mice following H1N1 influenza virus infection and implicated p63+Krt5+ distal airway stem cells, or DASCp63/Krt5, in this process. We show here that rare, preexisting DASCp63/K5 undergo a proliferative expansion in response to influenza and lineage-trace to nascent alveoli assembled at sites of interstitial inflammation. We also show that the ablation of DASCp63/Krt5 in vivo prevents the regeneration of lung tissue following influenza leading to pre-fibrotic lesions and deficient oxygen exchange. Finally, we demonstrate that exogenously cloned and propagated DASCp63/Krt5 readily contribute to lung regeneration following transplantation. The transplanted DASC ameliorated influenza-induced lung injury. These data suggest that DASCp63/K5 are required for lung regeneration and may have therapeutic utility in acute and chronic lung diseases. Transplanted DASC cells incorporated into damaged host lung were laser capture microdissected and analyzed. Duplicate mice were included. We used the Affymetrix Mouse Exon 1.0 ST platform

ORGANISM(S): Mus musculus

SUBMITTER: Frank McKeon 

PROVIDER: E-GEOD-60830 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Lung diseases such as chronic obstructive pulmonary disease and pulmonary fibrosis involve the progressive and inexorable destruction of oxygen exchange surfaces and airways, and have emerged as a leading cause of death worldwide. Mitigating therapies, aside from impractical organ transplantation, remain limited and the possibility of regenerative medicine has lacked empirical support. However, it is clinically known that patients who survive sudden, massive loss of lung tissue from necrotizing  ...[more]

Similar Datasets

2014-11-20 | E-GEOD-60829 | biostudies-arrayexpress
2014-11-20 | GSE60829 | GEO
2014-11-20 | GSE60830 | GEO
2014-11-20 | GSE60828 | GEO
2014-11-20 | E-GEOD-60828 | biostudies-arrayexpress
2014-11-20 | GSE61091 | GEO
2014-11-20 | E-GEOD-61091 | biostudies-arrayexpress
2021-03-10 | GSE168573 | GEO
2011-11-01 | E-GEOD-32606 | biostudies-arrayexpress
2011-11-01 | E-GEOD-32604 | biostudies-arrayexpress