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RNA-Seq in neurons from E16.5 mouse embryonic cortices from WT and SMCX KO mice

ABSTRACT: We conducted RNA-Seq (using Direct Ligation of Adapters to first strand cDNA) in neurons from E16.5 mouse embryonic cortices from WT and SMCX KO mice and harvested after 10 days in vitro culture. We sequenced RNA samples after 10 days in vitro cultures in biological and technical duplicates. 4 RNA samples from WT and SMCX KO neurons. We also sequenced RNA samples from same neurons after stimulation with KCl for 60 mins. So a total of 8 RNA-Seq samples.

ORGANISM(S): Mus musculus

SUBMITTER: Saurabh Agarwal

PROVIDER: E-GEOD-61024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A Mouse Model of X-linked Intellectual Disability Associated with Impaired Removal of Histone Methylation.

Iwase Shigeki S Brookes Emily E Agarwal Saurabh S Badeaux Aimee I AI Ito Hikaru H Vallianatos Christina N CN Tomassy Giulio Srubek GS Kasza Tomas T Lin Grace G Thompson Andrew A Gu Lei L Kwan Kenneth Y KY Chen Chinfei C Sartor Maureen A MA Egan Brian B Xu Jun J Shi Yang Y

Cell reports 20160121 5

Mutations in a number of chromatin modifiers are associated with human neurological disorders. KDM5C, a histone H3 lysine 4 di- and tri-methyl (H3K4me2/3)-specific demethylase, is frequently mutated in X-linked intellectual disability (XLID) patients. Here, we report that disruption of the mouse Kdm5c gene recapitulates adaptive and cognitive abnormalities observed in XLID, including impaired social behavior, memory deficits, and aggression. Kdm5c-knockout brains exhibit abnormal dendritic arbor ...[more]

PMID: 26804915

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