Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant


ABSTRACT: We expressed either a wt or a phosphomutant version of ZFP36L1 in IMR90 ER:RAS cells. 7 days upon RAS induction (when the cells reach a fully senescent phenotype) we collected the RNA. ZFP36L1 is a RNA binding protein that binds to AU-rich elements in the 3’UTR of mRNAs and triggers their degradation. Our previous experiments showed that the activity of ZFP36L1 was key in the regulation of the senescent phenotype.By performing RNAseq we have uncovered the effect of expressing a constitutively active mutant of ZFP36L1 within the senescent transcriptome. 4 samples examined: Non-senescent cells (EV - 4OHT), Senescent cells (EV + 4OHT), Senescent cells expressing ZFP36L1wt and Senescent cells expressing ZFP36L1mut

ORGANISM(S): Homo sapiens

SUBMITTER: Gopuraja Dharmalingam 

PROVIDER: E-GEOD-61130 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Senescent cells secrete a combination of factors collectively known as the senescence-associated secretory phenotype (SASP). The SASP reinforces senescence and activates an immune surveillance response, but it can also show pro-tumorigenic properties and contribute to age-related pathologies. In a drug screen to find new SASP regulators, we uncovered the mTOR inhibitor rapamycin as a potent SASP suppressor. Here we report a mechanism by which mTOR controls the SASP by differentially regulating t  ...[more]

Similar Datasets

2012-07-09 | E-GEOD-38442 | biostudies-arrayexpress
2015-07-07 | GSE61130 | GEO
2022-07-19 | GSE155904 | GEO
2009-06-02 | E-GEOD-15299 | biostudies-arrayexpress
| PRJNA834739 | ENA
2018-02-14 | GSE110581 | GEO
2012-07-31 | E-GEOD-30790 | biostudies-arrayexpress
2014-04-30 | E-GEOD-40104 | biostudies-arrayexpress
2020-11-20 | GSE146073 | GEO
2020-10-02 | GSE136180 | GEO