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Gene expression in WT and Ikaros-deficient LSK cells

ABSTRACT: The Ikaros zink finger transcription factor is a critical regulator of the hematopietic system, and plays an important role in the regulation of the development and function of several blood cell lineages. We used microarrays to characterize how Ikaros deficieny affects global transcription in the hematopoietic stem and progenitor cells from wild-type and Ikaros mutant mice. lin- Sca1+ c-Kit+ (LSK) cells, that contains the hematopoietic stem cells and the multipotent progenitor cells, were sorted by FACS from the bone marrow of 6-7 weeks old WT and IkL/L mutant mice. Total RNA extracted from these cells was subjected to transcriptome analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Attila Oravecz

PROVIDER: E-GEOD-61147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ikaros mediates gene silencing in T cells through Polycomb repressive complex 2.

Oravecz Attila A Apostolov Apostol A Polak Katarzyna K Jost Bernard B Le Gras Stéphanie S Chan Susan S Kastner Philippe P

Nature communications 20151109

T-cell development is accompanied by epigenetic changes that ensure the silencing of stem cell-related genes and the activation of lymphocyte-specific programmes. How transcription factors influence these changes remains unclear. We show that the Ikaros transcription factor forms a complex with Polycomb repressive complex 2 (PRC2) in CD4(-)CD8(-) thymocytes and allows its binding to more than 500 developmentally regulated loci, including those normally activated in haematopoietic stem cells and ...[more]

PMID: 26549758

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