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Expression Profiling E. Coli Evolved on Glucose Minimal Media

ABSTRACT: mRNA sequencing was performed on E. coli evolved at 37C on M9 Minimal Media with Glucose Growth of evolved E. coli was characterized under the same conditions as the evolution and compared to the wild type parent strain

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Bernhard Palsson

PROVIDER: E-GEOD-61327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium.

LaCroix Ryan A RA Sandberg Troy E TE O'Brien Edward J EJ Utrilla Jose J Ebrahim Ali A Guzman Gabriela I GI Szubin Richard R Palsson Bernhard O BO Feist Adam M AM

Applied and environmental microbiology 20141010 1

Adaptive laboratory evolution (ALE) has emerged as an effective tool for scientific discovery and addressing biotechnological needs. Much of ALE's utility is derived from reproducibly obtained fitness increases. Identifying causal genetic changes and their combinatorial effects is challenging and time-consuming. Understanding how these genetic changes enable increased fitness can be difficult. A series of approaches that address these challenges was developed and demonstrated using Escherichia c ...[more]

PMID: 25304508

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Expression Profiling E. Coli Evolved on Glucose Minimal Media

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