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RNA-sequencing study of peripheral blood monocytes for chronic periodontitis

ABSTRACT: We confirmed immune response as the key mechanism and provided solid evidence for novel genes (e.g., FCAR and CUX1) and distinct biological processes (e.g., endocytosis, cytokine production and apoptosis) as potentially new important factors/mechanisms contributing to chronic periodontitis pathogenesis. We performed an RNA-sequencing (RNA-seq) study of peripheral blood monocytes (PBMs) in 5 non-smoking moderate to severe CP (case) subjects vs. 5 controls. We replicated the DEx transcripts/isoforms using an independent microarray dataset. We also pathway-based analysis on the identified/replicated DEx transcripts/isoforms using DAVID performed (Database for Annotation, Visualization and Integrated Discovery).

ORGANISM(S): Homo sapiens

SUBMITTER: Yao-Zhong Liu

PROVIDER: E-GEOD-61490 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-sequencing study of peripheral blood monocytes in chronic periodontitis.

Liu Yao-Zhong YZ Maney Pooja P Puri Jyoti J Zhou Yu Y Baddoo Melody M Strong Michael M Wang Yu-Ping YP Flemington Erik E Deng Hong-Wen HW

Gene 20160123 2

<h4>Background</h4>Monocytes are an important cell type in chronic periodontitis (CP) by interacting with oral bacteria and mediating host immune response. The aim of this study was to reveal new functional genes and pathways for CP at monocyte transcriptomic level.<h4>Methods</h4>We performed an RNA-sequencing (RNA-seq) study of peripheral blood monocytes (PBMs) in 5 non-smoking moderate to severe CP (case) individuals vs. 5 controls. We took advantage of a microarray study of periodontitis to ...[more]

PMID: 26812355

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