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Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Eisen Christian

PROVIDER: E-GEOD-61861 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [mouse]

Project description:Isolation and culture of primary prostate epithelial stem/progenitor cells (PESC) has been proven difficult and ineffective. Here we present methods to grow and expand both murine and human basal PESCs long-term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-Sca1+ CD49f+Trop2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+Trop2high PESCs. The gene expression profiles of expanded basal PESCs show similarities to ES cells and Lamin B1 and PRDX1 were identified as novel PESC markers. If transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules demonstrating their stem cell activity in vivo. The novel methods will facilitate the cellular, molecular and genomic characterization of normal and pathologic prostate glands of mouse and human origin. Murine prostate cells were seperately cultured under spheroid and adherent stem cell conditions and subsequently used for RNA extraction

2015-02-20 | E-GEOD-61860 | biostudies-arrayexpress

Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [human]

Project description:Isolation and culture of primary prostate epithelial stem/progenitor cells (PESC) has been proven difficult and ineffective. Here we present methods to grow and expand both murine and human basal PESCs long-term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin-Sca1+ CD49f+Trop2high phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin-CD49f+Trop2high PESCs. The gene expression profiles of expanded basal PESCs show similarities to ES cells and Lamin B1 and PRDX1 were identified as novel PESC markers. If transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules demonstrating their stem cell activity in vivo. The novel methods will facilitate the cellular, molecular and genomic characterization of normal and pathologic prostate glands of mouse and human origin. Human prostate cells derived from two individual patients were seperately cultured under spheroid and adherent stem cell conditions and subsequently used for RNA extraction

2015-02-20 | E-GEOD-61859 | biostudies-arrayexpress

Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin.

Project description:Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin(-)SCA-1(+)CD49f(+)TROP2(high) phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin(-)CD49f(+)TROP2(high) PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.

| S-EPMC4375832 | biostudies-literature

Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-02-20 | GSE61861 | GEO

Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [mouse]

2015-02-20 | GSE61860 | GEO

Defined conditions for the isolation and expansion of basal prostate stem cells of mouse and human origin [human]

2015-02-20 | GSE61859 | GEO

Lambda light chain knockdown in ALMC1 human myeloma cells

Project description:We used siRNA to knockdown lambda light chain expression in ALMC1 cells that express an intact IgG lambda monoclonal protein. We compared knockdown cells to cells treated with control siRNA.

2014-01-30 | E-GEOD-54507 | biostudies-arrayexpress

Transcriptomics response to miR-644 overexpression in three breast cancer cell lines

Project description:The goal of the study is to investigate the effect of microRNA-644 overexpression by chemical mimics on gene expression in breast cancer cell lines. To this purpose control and microRNA-644 mimics are used to transfect MDA-MB-231 cells, SK-BR-3 cells, and MCF-7 cells respectively. Transcriptomics profiling was performed with Illumina HumanHT-12 V4.0 BeadChip microarray. Three cell lines are treated with scramble microRNA mimic (control herein) and microRNA-644 mimic (miR-644 herein), with two replicates per condition. mRNA expression is compared between miR-644 and control treatment within each cell line, respectively.

2016-07-25 | E-GEOD-82058 | biostudies-arrayexpress

Expansion of multipotent stem cells from the adult human brain

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-19 | E-GEOD-41470 | biostudies-arrayexpress

Autonomous extracellular matrix remodeling controls a progressive adaptation in muscle stem cell regenerative capacity during development

Project description:Muscle stem cells (MuSC) exhibit distinct behaviors during successive phases of developmental myogenesis. However, how their transition to adulthood is regulated is poorly understood. Here we show that fetal MuSC resist progenitor specification and exhibit altered division dynamics, intrinsic features that are progressively lost postnatally. Following transplantation, fetal MuSC more efficiently expand and contribute to muscle repair. Conversely, the efficiency of niche colonization increases in adulthood, indicating a balance between muscle growth and stem cell pool repopulation. Gene expression profiling identified several extracellular matrix (ECM) molecules preferentially expressed in fetal MuSC, including tenascin-C, fibronectin and collagen VI. Loss-of-function experiments confirmed their essential and stage-specific role in regulating MuSC function. Finally, fetal-derived paracrine factors were able to enhance adult MuSC regenerative potential. Together, these findings demonstrate that MuSC change the way in which they remodel their microenvironment to direct stem cell behavior in support of the unique demands of muscle development or repair. MuSCs were isolated through fluorescent-activate cell sorting usinf alpha7-integirn and CD34 as markers to identify the cell population. Total mRNA was then isolated, and samples at different developmental times were compared.

2016-01-20 | E-GEOD-76989 | biostudies-arrayexpress

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