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Transcription profiling of human HepG2 cell line overexpressing miR-124 sampled at multiple times points to study the targets and functions of miR-124

ABSTRACT: This time course analysis is to study the targets and functions of miR-124. MiR-124 was overexpressed and the effect of miR-124 overexpression was compared to negative controls to identify downregulated genes and potential miRNA targets. Experiment Overall Design: one array for each time point of miR-124 and negative control transfections

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaowei Wang

PROVIDER: E-GEOD-6207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic identification of microRNA functions by combining target prediction and expression profiling.

Wang Xiaowei X Wang Xiaohui X

Nucleic acids research 20060320 5

Target predictions and validations are major obstacles facing microRNA (miRNA) researchers. Animal miRNA target prediction is challenging because of limited miRNA sequence complementarity to the targets. In addition, only a small number of predicted targets have been experimentally validated and the miRNA mechanism is poorly understood. Here we present a novel algorithm for animal miRNA target prediction. The algorithm combines relevant parameters for miRNA target recognition and heuristically a ...[more]

PMID: 16549876

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