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RIP-seq of polyribosomal SLBP RNP

ABSTRACT: UV cross-linking and immunoprecipiatation with high throughput sequencing of cytoplasmic SLBP RNP Polyclonal Î±-SLBP antibody was used to immunoprecipitate SLBP RNP from polyribosomal fractions of HeLa S3 lysates. Mock immunoprecipitation were also performed and serve as a negative control.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Whitfield

PROVIDER: E-GEOD-62076 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A multiprotein occupancy map of the mRNP on the 3' end of histone mRNAs.

Brooks Lionel L Lyons Shawn M SM Mahoney J Matthew JM Welch Joshua D JD Liu Zhongle Z Marzluff William F WF Whitfield Michael L ML

RNA (New York, N.Y.) 20150916 11

The animal replication-dependent (RD) histone mRNAs are coordinately regulated with chromosome replication. The RD-histone mRNAs are the only known cellular mRNAs that are not polyadenylated. Instead, the mature transcripts end in a conserved stem-loop (SL) structure. This SL structure interacts with the stem-loop binding protein (SLBP), which is involved in all aspects of RD-histone mRNA metabolism. We used several genomic methods, including high-throughput sequencing of cross-linked immunoprec ...[more]

PMID: 26377992

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