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Identification of the BRD1 interactome and its impact on schizophrenia risk

ABSTRACT: We identified protein-protein interactions and chromatin binding sites for two isforms of BRD1 (BRD1-S and BRD1-L) using Co-IP MS/MS and ChIP-seq. BRD1 isoforms were cloned, epitope-tagged (pcDNA 6 V5-His6, Lifetechnologies) and stably expressed in HEK293T cells. Chromatin immunoprecipitations were performed using anti V5-antibody conjugated agarose beads (Sigma-Aldrich) and anti HA antibody conjugated agarose beads (Sigma-Aldrich) as controls

ORGANISM(S): Homo sapiens

SUBMITTER: Tue Fryland

PROVIDER: E-GEOD-62811 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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