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The DREAM complex promotes gene body deposition of H2A.Z for target repression

ABSTRACT: As part of a study of the function of the C. elegans DREAM complex, here we have used ChIP-seq to analyze the patterns of histone variant HTZ-1/H2A.Z and various histone modifications in lin-35(n745) mutants. In addition, we have used RNA-seq to analyze gene expression in lin-35 and htz-1 mutants as compared with wild-type L3 larvae. EXPERIMENT TYPE: CHIP-seq, RNA-seq. BIOLOGICAL SOURCE: Strain: N2, JA1507, VC2490; Developmental Stage: L3 Larva; Genotype: wild type, lin-35(n745), htz-1(ok3099); Sex: mixed Male and Hermaphrodite population; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temp (temperature) 20 degree celsius; Antibodies: HTZ-1 JA00001, H3K4me3 Wako 305-34819, H4ac4 Active Motif 39177, Histone H3 Abcam ab1791

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Przemyslaw Stempor

PROVIDER: E-GEOD-62833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The DREAM complex promotes gene body H2A.Z for target repression.

Latorre Isabel I Chesney Michael A MA Garrigues Jacob M JM Stempor Przemyslaw P Appert Alex A Francesconi Mirko M Strome Susan S Ahringer Julie J

Genes & development 20150301 5

The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual pattern of high gene body HTZ-1/H2A.Z. In mutants of lin-35, the sole p130/Rb-like gene in C. elegans, DREAM targets have reduced gene body HTZ-1/H2A.Z and increased expression. Consistent with a repressive role for gene body H2A.Z, many DREAM targets ar ...[more]

PMID: 25737279

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