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Polysome profiling of glp-4 mutant animals

ABSTRACT: To determine the translational status of mRNAs in glp-4 mutant animals, we analyzed total and polysomal RNA levels by tiling arrays A total of 8 samples was analyzed (quadruplicates of glp-4_total and glp-4_polysomal, respectively)

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Dimos Gaidatzis

PROVIDER: E-GEOD-62860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional characterization of C. elegans Y-box-binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes.

Arnold Andreas A Rahman Md Masuder MM Lee Man Chun MC Muehlhaeusser Sandra S Katic Iskra I Gaidatzis Dimos D Hess Daniel D Scheckel Claudia C Wright Jane E JE Stetak Attila A Boag Peter R PR Ciosk Rafal R

Nucleic acids research 20141105 21

The cold shock domain is one of the most highly conserved motifs between bacteria and higher eukaryotes. Y-box-binding proteins represent a subfamily of cold shock domain proteins with pleiotropic functions, ranging from transcription in the nucleus to translation in the cytoplasm. These proteins have been investigated in all major model organisms except Caenorhabditis elegans. In this study, we set out to fill this gap and present a functional characterization of CEYs, the C. elegans Y-box-bind ...[more]

PMID: 25378320

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Polysome profiling of glp-4 mutant animals

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