Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq for Thermotoga maritima MSB8 glucose evolved cultures


ABSTRACT: RNA-seq was performed on T. maritima wild type, three glucose evolved cultures, and three glycerol adapted cultures. Wild type and glucose evolved strains were grown on glucose minimal media and glycerol evolved cultures were grown on glycerol minimal media. All samples were harvested in exponential phase. Seven samples are included with two biological replicates for each condition.

ORGANISM(S): Thermotoga maritima MSB8

SUBMITTER: Haythem Latif 

PROVIDER: E-GEOD-63141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Adaptive Evolution of Thermotoga maritima Reveals Plasticity of the ABC Transporter Network.

Latif Haythem H   Sahin Merve M   Tarasova Janna J   Tarasova Yekaterina Y   Portnoy Vasiliy A VA   Nogales Juan J   Zengler Karsten K  

Applied and environmental microbiology 20150605 16


Thermotoga maritima is a hyperthermophilic anaerobe that utilizes a vast network of ABC transporters to efficiently metabolize a variety of carbon sources to produce hydrogen. For unknown reasons, this organism does not metabolize glucose as readily as it does glucose di- and polysaccharides. The leading hypothesis implicates the thermolability of glucose at the physiological temperatures at which T. maritima lives. After a 25-day laboratory evolution, phenotypes were observed with growth rates  ...[more]

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