Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

CX3CR1/Fractalkine receptor expression separates memory CD8+ T cells with distinct functional profiles

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Joachim Schultze

PROVIDER: E-GEOD-63147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional classification of memory CD8(+) T cells by CX3CR1 expression.

Böttcher Jan P JP Beyer Marc M Meissner Felix F Abdullah Zeinab Z Sander Jil J Höchst Bastian B Eickhoff Sarah S Rieckmann Jan C JC Russo Caroline C Bauer Tanja T Flecken Tobias T Giesen Dominik D Engel Daniel D Jung Steffen S Busch Dirk H DH Protzer Ulrike U Thimme Robert R Mann Matthias M Kurts Christian C Schultze Joachim L JL Kastenmüller Wolfgang W Knolle Percy A PA

Nature communications 20150925

Localization of memory CD8(+) T cells to lymphoid or peripheral tissues is believed to correlate with proliferative capacity or effector function. Here we demonstrate that the fractalkine-receptor/CX3CR1 distinguishes memory CD8(+) T cells with cytotoxic effector function from those with proliferative capacity, independent of tissue-homing properties. CX3CR1-based transcriptome and proteome-profiling defines a core signature of memory CD8(+) T cells with effector function. We find CD62L(hi)CX3CR ...[more]

PMID: 26404698

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Project description:Pericryptal myofibroblasts in the colon and rectum play an important role in regulating the normal colorectal stem cell niche and facilitating tumour progression. Myofibroblasts have previously mostly been distinguished from normal fibroblasts only by the expression of Î± smooth muscle actin (Î±SMA). We now identify AOC3, a surface monoamine oxidase, as a new marker of myofibroblasts by showing that it is the target protein of the myofibroblast reacting monoclonal antibody (mAb), PR2D3. The normal and tumour tissue distribution and the cell line reactivity of AOC3 match that expected for myofibroblasts. We have shown that the surface expression of AOC3 is sensitive to digestion by trypsin and collagenase and that anti-AOC3 antibodies can be used for FACS sorting of myofibroblasts obtained by non-enzymatic procedures. Whole genome microarray mRNA expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly expressed differentially between these two cell types; NKX2-3 and LRRC17 are expressed in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts. Transforming Growth Factor Î² (TGFÎ²) substantially down-regulated AOC3 expression in myofibroblasts but not in skin fibroblasts, in which it dramatically increased the expression of Î±SMA. A knockdown of NKX2-3 in myofibroblasts caused a decrease of myofibroblast-related gene expression and an increased expression of the fibroblast associated gene, SHOX2, suggesting that NKX2-3 is a key mediator for maintaining myofibroblast characteristics. Our results show that colorectal myofibroblasts, as defined by the expression of AOC3, NKX2-3 and other markers, are a distinctly different cell type from TGFÎ² activated fibroblasts. colorectal myofibroblast specific markers and expression profiles were sought by comparing four primary myofibroblast cultures to a panel of four dermal and foreskin fibroblast cell lines Four primary myofibroblast cultures established from adult human colon compared to four skin fibroblast cell lines to identify intestinal myofibroblast specific markers

Dataset Information

CX3CR1/Fractalkine receptor expression separates memory CD8+ T cells with distinct functional profiles

Publications

Functional classification of memory CD8(+) T cells by CX3CR1 expression.

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