Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Phytophthora infestans

SUBMITTER: Anna Åsman

PROVIDER: E-GEOD-63293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection.

Åsman Anna K M AK Vetukuri Ramesh R RR Jahan Sultana N SN Fogelqvist Johan J Corcoran Pádraic P Avrova Anna O AO Whisson Stephen C SC Dixelius Christina C

BMC microbiology 20141210

<h4>Background</h4>The oomycete Phytophthora infestans possesses active RNA silencing pathways, which presumably enable this plant pathogen to control the large numbers of transposable elements present in its 240 Mb genome. Small RNAs (sRNAs), central molecules in RNA silencing, are known to also play key roles in this organism, notably in regulation of critical effector genes needed for infection of its potato host.<h4>Results</h4>To identify additional classes of sRNAs in oomycetes, we mapped ...[more]

PMID: 25492044

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Project description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana (Hybridisations done at NASC); The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Series 2: Base line experiment for pathogen infection. Growth and infection conditions: Seeds will be stratified for 3 days at 4Â°C and sown on soil. Plants are grown at 22Â°C under a 8/16 hour light/dark regime in Percival growth chambers. All pathogen treatments will be performed on leaves of 5-weeks old plants. Bacterial infiltrations will be performed with 10-8 cfu/ml in 10 mM MgCl2. 5x105 spores of Phytophthora infestans in water will be applied to leaf surfaces. LPS (100 Âµg/ml), HrpZ (1 ÂµM), NPP1 (2 ÂµM) or flagellin (1 ÂµM flg22) will be applied in water. Experiments should be performed in triplicate at the time points indicated below. Experimenter name = Dierk Scheel , Frederic Brunner , Lore Westphal; Experimenter institute = AtGenExpress Experiment Overall Design: 18 samples were used in this experiment

Dataset Information

Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection

Publications

Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection.

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