Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Colon gene expression signatures in mice mono-associated with IBD-associated micro-organisms

ABSTRACT: Studying host-microbiota interactions is fundamental to understand mechanisms involved in intestinal inflammation and inflammatory bowel diseases. In this work, we studied these interactions in mice mono-associated with 4 bacteria and 2 yeasts, all representative of intestinal microbiota and/or associated with IBD pathogenesis: Bacteroides thetaiotaomicron, adhesive-invasive Escherichia coli (AIEC), Ruminococcus gnavus, Roseburia intestinalis, Saccharomyces boulardii and Candida albicans. Transcriptomics analyses showed that B. thetaiotaomicron had the highest immunological effect, being able to almost recapitulate the effects of a whole microbiota, and particularly induced Treg pathways. Furthermore, this analysis also pointed out the effects of E. coli AIEC LF82 on IDO activation and of S. boulardii on angiogenesis, as well as major effects of R. gnavus on metabolism. This work therefore reveals information on the role of each micro-organism and proposes several tracks to follow to better understand IBD pathogenesis and identify therapeutic targets 6 mono-associations + 2 controls (germ-free and conventionalized mice), with 5 to 7 mice per group.

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Hoffmann

PROVIDER: E-GEOD-63299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microorganisms linked to inflammatory bowel disease-associated dysbiosis differentially impact host physiology in gnotobiotic mice.

Hoffmann Thomas W TW Pham Hang-Phuong HP Bridonneau Chantal C Aubry Camille C Lamas Bruno B Martin-Gallausiaux Camille C Moroldo Marco M Rainteau Dominique D Lapaque Nicolas N Six Adrien A Richard Mathias L ML Fargier Emilie E Le Guern Marie-Emmanuelle ME Langella Philippe P Sokol Harry H

The ISME journal 20150728 2

Studying host-microbiota interactions are fundamental to understanding the mechanisms involved in intestinal homeostasis and inflammation. In this work, we analyzed these interactions in mice that were mono-associated with six microorganisms that are representative of inflammatory bowel disease (IBD)-associated dysbiosis: the bacteria Bacteroides thetaiotaomicron, adhesive-invasive Escherichia coli (AIEC), Ruminococcus gnavus and Roseburia intestinalis; a yeast used as a probiotic drug, Saccharo ...[more]

PMID: 26218241

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Dataset Information

Colon gene expression signatures in mice mono-associated with IBD-associated micro-organisms

Publications

Microorganisms linked to inflammatory bowel disease-associated dysbiosis differentially impact host physiology in gnotobiotic mice.

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