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Separated and combined effects of the over-expression of a plant defensin and of a 20 mM Zn treatment on yeast.

ABSTRACT: The expression of plant defensins in Saccharomyces cerevisiae has been shown to increase tolerance to Zn of yeast (Mirouze et al., 2006; Shahzad et al., 2013). The present transcriptomic approach has been conducted to uncover the mechanism underlying this phenotype. WT yeast was compared to yeast expressing the AhPFF1.1b plant defensin when grown in both control medium and medium supplemented with 20 mM ZnSO4. 4 conditions with 2 biological replicates compared: control yeast (BY4741) grown on control medium, control yeast grown in the presence of 20 mM ZnSO4, yeast expressing the AhPDF1.1b defensin grown in control medium, yeast expressing the AhPDF1.1b defensin grown in the presence of 20 mM ZnSO4

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Pierre BERTHOMIEU

PROVIDER: E-GEOD-63395 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Separated and combined effects of the over-expression of a plant defensin and of a 20 mM Zn treatment on yeast.

Project description:The expression of plant defensins in Saccharomyces cerevisiae has been shown to increase tolerance to Zn of yeast (Mirouze et al., 2006; Shahzad et al., 2013). The present transcriptomic approach has been conducted to uncover the mechanism underlying this phenotype. WT yeast was compared to yeast expressing the AhPFF1.1b plant defensin when grown in both control medium and medium supplemented with 20 mM ZnSO4.

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Project description:We observed the expression profile of the total mRNA of litR (TTHB100) deletion mutant of T. thermophilus HB8 strain grown in a rich (TM) medium at 60 M-BM-0C under the dark condition compared with that of wild-type. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 60 M-BM-0C for 16 hours in 100 mL of TM medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.5 with NaOH. The cells (1 mL) were inoculated into 100 mL of the synthetic medium and then cultivated at 60M-BM-0C for 8 hours under dark condition. Then the 1.0 mM of ZnSO4 was added into the medium, and continued cultivation. Cells were collected, and then, the expression of each mRNA was analyzed.

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Project description:Analysis of mid-term response to hydroxyurea in a yeast wild-type strain (W303a). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Gene expression changes were analysed compared to the same strain grown in the same medium without HU. Keywords: repeat sample

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Streptococcus pneumoniae

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Project description:Analysis of the response to hydroxyurea in a yeast yap1 mutant strain compared to wild-type strain (BY4741 or BY4742 backgrounds). Cells were grown in YPD rich medium containing 200 mM hydroxyurea (HU) for 2 hours. Keywords: repeat sample

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