Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

39 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from persister and normal cells of Pseudomonas aeruginosa PAO1

ABSTRACT: Pseudomonas aeruginosa PAO1 persister and normal cells were treated with and without Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) to understand the effect of GM-CSF on gene expression of PAO1. We used DNA microarrays to identify the down-regulated and up-regulated genes after GM-CSF treatment. Pseudonmonas aeruginosa PAO1 normal cells were obtained from stationary phase culture and persister cells were isolated by lysing normal cells with ciprofloxacin. The normal cells and isolated persister cells were treated with GM-CSF and selected for RNA extraction and hybridization on Affymetrix microarrays. The experiment was performed in two biological replicates. The samples for the biological replciates were: Control C1, Treatment T1, Control C2, and Treatment T2.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Geetika Choudhary

PROVIDER: E-GEOD-63588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from persister and normal cells of Pseudomonas aeruginosa PAO1

Project description:Pseudomonas aeruginosa PAO1 persister and normal cells were treated with and without Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) to understand the effect of GM-CSF on gene expression of PAO1. We used DNA microarrays to identify the down-regulated and up-regulated genes after GM-CSF treatment.

2014-11-25 | GSE63588 | GEO

Effects of (E)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one (BF8) on P. aeruginosa PAO1 persister cells (three biological replicates)

Project description:Twenty eight genes in PAO1 persister cells were consistently induced by treatment with 1 ug/mL BF8 for 1 h. Persister cells were isolated from PAO1 overnight cultures and treated with and without BF8 for microarray analysis.

2012-03-24 | E-GEOD-36753 | biostudies-arrayexpress

Effects of (E)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one (BF8) on E. coli RP437 persister cells (two biological replicates)

Project description:Eighteen genes in RP437 persister cells were consistently induced/decreased by treatment with 5 Âµg/mL BF8 for 1 h. Persister cells were isolated from RP437 overnight cultures and treated with and without BF8 for microarray analysis.

2013-02-13 | E-GEOD-44273 | biostudies-arrayexpress

Pseudomonas aeruginosa

Project description:Expression data from persister and normal cells of Pseudomonas aeruginosa PAO1

| PRJNA268347 | ENA

Transcriptional response to 2-oxoglutarate (alpha-ketoglutarate) in Pseduomonas aeruginosa PAO1

Project description:The transcriptome of P. aeruginosa PAO1 in the presence of extracelluar 2-oxoglutarate at a concentration of 20 mM. We determined the transcriptional response of P. aeruignosa PAO1 to extracellular 2-oxoglutarate. P. aeruginosa PAO1 was grown in nutrient broth (Oxoid number 2) and induced with 20 mM 2-oxoglutarate. At 30 min post induction, total RNA was isolated and prepped for Affymetrix GeneChips.

2014-02-15 | E-GEOD-54032 | biostudies-arrayexpress

Expression of RpoN molecular roadblock in Pseudomonas aeruginosa PAO1 in rich media

Project description:The bacterial transcription factor RpoN regulates an extensive network of genes whose products are involved in diverse biological functions. We constructed a small peptide termed the RpoN molecular roadblock, which binds to and blocks transcription from RpoN promoters. This RpoN molecular roadblock can be used in any bacterium to obtain information on the RpoN regulon. We expressed the RpoN molecular roadblock in P. aeruginosa PAO1 and used microarrays to identify genes that were differentially transcribed due to the RpoN molecular roadblock. The RpoN molecular roadblock was expressed in P. aeruginosa PAO1 in mid-exponential phase in rich media. Total RNA was isolated and prepped for Affymetrix GeneChips.

2012-02-09 | E-GEOD-35632 | biostudies-arrayexpress

Pseudomonas aeruginosa gene expression in artificial sputum medium using a broad capture array designated the PANarray

Project description:The PANarray design (GPL13324) contains the genes of eight P. aeruginosa genomes in non-redundant format, thus allowing identification of expression of non-PAO1 and other P. aeruginosa genes. For the series GSE28152, isogenic isolates were sequentially collected from two cystic fibrosis (CF) patients several years apart. The isolates had not been eradicated in the meantime and represent persister strains. One was an Australian Epidemic Strain-1 isolate and the other a non-epidemic strain. Strains were cultured in an artificial sputum medium (ASMDM) closely resembling CF sputum.

2011-07-15 | E-GEOD-28152 | biostudies-arrayexpress

Transcription profiling of P. aeruginosa PAO1 PA2663 mutant vs. wild type strain

Project description:P. aeruginosa PAO1 wild type and PA2663 mutant strains expression in biofilm cells relative to P. aeruginosa PAO1 wild type strain expression in biofilm cells. All samples cultured in LB with glass wool Experiment Overall Design: Strains: P. aeruginosa PAO1 wild-type, PA2663 mutant Experiment Overall Design: Medium: LB Experiment Overall Design: Biofilm grown on glass wool Experiment Overall Design: Time: 24 h Experiment Overall Design: Cell Type: biofilm

2008-04-07 | E-GEOD-9255 | biostudies-arrayexpress

Regulon of transcriptional regulator PA2449 in Pseduomonas aeruginosa PAO1

Project description:The putative trancriptional regulator PA2449 was found to be essential for both glycine/serine metabolism and the production of phenazines in P. aeruignosa PAO1. We examined the regulon controlled by PA2449 via microarray analysis between wild-type P. aeruignosa PAO1 and the PA2449-null mutant P. aeruginosa PW5126. Both strains were obtained from the PA-two allele library (Univ. of Washington, Ref. Jacobs et al. 2003. PNAS 100, 14339). Strains were grown under conditions known to induce phenazine biosynthesis (peptone broth), and their resulting transcriptomes were compared. Total RNA was isolated and prepped for Affymetrix GeneChips.

2012-10-15 | E-GEOD-39044 | biostudies-arrayexpress

mRNA profiling on GM-CSF knockout CART19 compared to wildtype CART19 and Ctrl gRNA CART19 produced from normal donors

Project description:RNA sequencing was performed on three biological replicates of CART19 (GM-CSF wildtype), GM-CSF knockout CART19, and Ctrl gRNA CART19 produced from normal donors.

2022-04-15 | GSE147991 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data