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Short and Long RNA sequencing of human mature erythrocytes

ABSTRACT: We employed high-throughput sequencing of both short (~18-24nt) and long (>200nt) RNAs in human erythrocytes. We obtained blood from five healthy individuals for the short (small) RNA-seq library preparations and blood from three individuals for the long RNA-seq library preparations. We identified an abundant, diverse population of RNAs. Both polyadenylated and nonpolyadenlated long RNAs were identified. Additionally, known and novel microRNAs were identified in the short RNA dataset using the probabalistic modeling algorithm miRDeep. These RNAs lend insight into erythrocyte biology and provide utility as potential biomarkers. To determine both shared and unique aspects of the erythrocyte long RNA transcriptome, we compared this transcriptome with that of the PBMC and CD34+ erythroid progenitor transcriptomes. Sequencing of RNAs from mature erythrocytes of healthy individuals

ORGANISM(S): Homo sapiens

SUBMITTER: Jen-Tsan Chi

PROVIDER: E-GEOD-63703 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes.

Doss Jennifer F JF Corcoran David L DL Jima Dereje D DD Telen Marilyn J MJ Dave Sandeep S SS Chi Jen-Tsan JT

BMC genomics 20151116

<h4>Background</h4>Human erythrocytes are terminally differentiated, anucleate cells long thought to lack RNAs. However, previous studies have shown the persistence of many small-sized RNAs in erythrocytes. To comprehensively define the erythrocyte transcriptome, we used high-throughput sequencing to identify both short (18-24 nt) and long (>200 nt) RNAs in mature erythrocytes.<h4>Results</h4>Analysis of the short RNA transcriptome with miRDeep identified 287 known and 72 putative novel microRNA ...[more]

PMID: 26573221

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