Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA profiling of two endothelial cells (MPEC and AKT-HUVEC) and one human fibroblast line (BJ)

ABSTRACT: Analysis of overall gene expression in endothelial cells, MPEC and AKT-HUVEC, comparing to a human fibroblast line, BJ. We are looking for the highly abundant genes in endothelial cells comparing to fibroblasts. Total RNA isolated from MPEC, AKT-HUVEC and BJ Fig 3b in publication.

ORGANISM(S): Homo sapiens

SUBMITTER: Der-I Kao

PROVIDER: E-GEOD-63843 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA profiling of two endothelial cells (MPEC and AKT-HUVEC) and one human fibroblast line (BJ)

Project description:Analysis of overall gene expression in endothelial cells, MPEC and AKT-HUVEC, comparing to a human fibroblast line, BJ. We are looking for the highly abundant genes in endothelial cells comparing to fibroblasts.

2014-12-04 | GSE63843 | GEO

RNA profiling of PDX1+ pancreatic progenitors before or after co-culture with different types of cells

Project description:Analysis of gene expression of Pdx-EGFP1+ pancreatic progenitors before or after co-culture at mRNA level. The hypothesis tested in the study was that the overall gene expression in Pdx1-EGFP+ does not alter after co-culture with endothelial cells. The result supported our hypothesis. Total RNA isolated from Pdx1-EGFP+ progenitors from the Pdx1-EGFP HUES8 cell-derived pancreatic progenitor population before (none) and after co-culture (AKT-HUVEC, MPEC, or BJ) Fig 2d in publication.

2014-12-04 | E-GEOD-63840 | biostudies-arrayexpress

RNA profiling of two endothelial cells (MPEC and AKT-HUVEC) and one human fibroblast line (BJ)

Project description:RNA profiling of two endothelial cells (MPEC and AKT-HUVEC) and one human fibroblast line (BJ)

| PRJNA269242 | ENA

Copy number variation between BJ cells and BJ-derived iPS cells

Project description:Copy number variation analysis between BJ cells and BJ cells derived iPS cells which were established by using non-transmissible measles virus vector

2016-06-22 | GSE79604 | GEO

Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells

Project description:The PI3K/Akt signaling pathway impacts various aspects of CD8 T cell homeostasis, such as effect versus memory cell differentiation, during viral infection. We used microarrays to determine which downstream molecules were affected and what other signaling pathways were interconnected with the Akt pathway by constitutive activation of Akt in LCMV-infected CD8 T cells. Splenocytes from naive P14/WT or P14/Akt mice were stained with anti-CD8 and anti-Ly5.1, and CD8 T cells were sorted using a FACSAria II instrument. Purified Ly5.1+ CD8 T cells from P14/WT or P14/Akt mice were transferred into B6 mice, which were subsequently infected with LCMV Armstrong. At day 8 post infection, splenocytes were stained with anti-CD8, anti-Ly5.1, anti-KLRG1, and anti-CD127. Following staining, short-lived effector cells (SLECs) and memory precursor effector cells (MPECs) were sorted using the FACSAria II instrument; the purity of the sorted cells was >95%. A total of 5 samples were analyzed, including WT naive, WT SLEC, WT MPEC, Akt naive and Akt SLEC.

2012-03-01 | E-GEOD-36168 | biostudies-arrayexpress

Nesodiprion zhejiangensis nucleopolyhedrovirus BJ strain:BJ

Project description:Nesodiprion zhejiangensis nucleopolyhedrovirus BJ strain:BJ Genome sequencing and assembly

| PRJNA994981 | ENA

Human BJ fibroblasts containing control vs. BRD7 shRNA

Project description:Transcriptional profiling of human BJ fibroblasts comparing control FF shRNA expressing cells vs. BRD7 shRNA expressing cells under two conditions, either untreated or treated with 8uM nutln-3a for 8 hours. This experiment was done using two independent shRNAs targeting BRD7. Nutlin-3a was used to stabilize p53 and induce its transcriptional activity.

2010-07-01 | GSE22607 | GEO

Effect of CCAR2 depletion on the gene expression profile of BJ-hTERT cells

Project description:CCAR2 is a nuclear protein recently emerged as a pivotal player of the DNA damage response since it has been found involved in both apoptosis induction and DNA repair. Differently, its role in tumorigenesis and cancer progression is still elusive. In our studies we found that CCAR2 depletion impairs the proliferation of human cancer cell lines, but leaves unaffected the growth of normal immortalized cells. To better investigate this point we performed a genome wide gene expression analyses in U2OS and BJ-hTERT depleted of CCAR2 and we found that loss of this protein causes the deregulation of genes implicated in the AKT pathway specifically in U2OS cells, but not in BJ-hTERT. In accordance with these results we found a reduction in AKT activation in all the tested cancer cell lines depleted of CCAR2, but not in the normal ones. The defective activation of AKT is caused by the upregulation of TRB3 gene in cancer cells depleted of CCAR2 and finally results in the reduction of GSK3β phosphorylation, prevention of G1/S transition and inhibition of cancer cell growth.

2017-03-08 | GSE87362 | GEO

H3K9me3 ChIP-Seq in BJ-HELT cells

Project description:We generated H3K9me3 profile of reads and peaks of BJ fibroblasts expressing hTERT and SV40 early region (BJ-HELT cell line).

2018-08-04 | GSE102278 | GEO

Bj Paraquat

Project description:This SuperSeries is composed of the following subset Series: GSE26236: Bj Paraquat fulminant shock vs. non-treatment GSE26252: Bj Paraquat prolonged exposure vs. non-treatment Refer to individual Series

2011-05-15 | E-GEOD-26531 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data