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Toxicogenomic responses in rainbow trout hepatocytes exposed to model chemicals and a synthetic mixture


ABSTRACT: Comparison of gene expression of exposed versus non-exposed Oncorhynchus mykiss hepatocytes to four model chemicals and a synthetic mixture. Hepatocytes were exposed for 24 hours to a single chemical and a synthetic mixture of 10 nM 17 alpha-ethinylestradiol (EE2), 0.75 nM 2,3,7,8-tetrachloro-di-benzodioxin (TCDD), 100 μM paraquat and 0.75 μM 4-nitroquinoline-1-oxide (NQO). Four biological replicates for both exposed and non-exposed Oncorhynchus mykiss hepatocytes with corresponding dye flips.

ORGANISM(S): Oncorhynchus mykiss

SUBMITTER: Eivind Finne 

PROVIDER: E-GEOD-6416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Toxicogenomic responses in rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to model chemicals and a synthetic mixture.

Finne E F EF   Cooper G A GA   Koop B F BF   Hylland K K   Tollefsen K E KE  

Aquatic toxicology (Amsterdam, Netherlands) 20070104 3


As more salmon gene expression data has become available, the cDNA microarray platform has emerged as an appealing alternative in ecotoxicological screening of single chemicals and environmental samples relevant to the aquatic environment. This study was performed to validate biomarker gene responses of in vitro cultured rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to model chemicals, and to investigate effects of mixture toxicity in a synthetic mixture. Chemicals used for 24h single  ...[more]

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