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Transcription profiling of Rattus norvegicus pancreatic beta cell line expressing HNF1b, HNF4a2 or HNF6

ABSTRACT: This SuperSeries is composed of the following subset Series:; GSE1589: Targets of HNF1b, HNF4a2 and HNF6 in INS-1 cells; GSE1590: Targets of HNF1b mutants in INS-1; GSE1591: INS-1 cell lines (FLP-In T-REx) Experiment Overall Design: Refer to individual Series

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ludger Klein-Hitpass

PROVIDER: E-GEOD-6428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.

Thomas Heike H Senkel Sabine S Erdmann Silke S Arndt Tanja T Turan Gülüzar G Klein-Hitpass Ludger L Ryffel Gerhart U GU

Nucleic acids research 20041101 19

Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell clones. Additionally, the clones contain the tetracycline repressor allowing tetracycline induction of the transgene. By oligonucleotide microarray we define the ...[more]

PMID: 15520459

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