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HP1? has distinct subnuclear localizations, chromatin binding features and functions in embryonic stem cells and differentiating cells [expression]

ABSTRACT: We used microarrays to identify the gene expression changes in Cbx1-/- (HP1beta) knockout embryonic stem cells (ESCs) and Cbx5-/- (HP1alpha) knockout ESCs compared to WT ESCs and in embryoid bodies (EBs) differentiated from those three ESC types. ESCs were grown and the pluripotent SSEA1-positive cells from all ESC types using MACS were sorted and harvested or sorted and differentiated into EBs. Total RNA from all samples was extracted.

ORGANISM(S): Mus musculus

SUBMITTER: Eran Meshorer

PROVIDER: E-GEOD-65121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from Smarcd1 knockdown R1 embryonic stem cells

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HP1β has distinct subnuclear localizations, chromatin binding features and functions in embryonic stem cells and differentiating cells [expression]

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Polycomb protein EED is required for selective silencing of pluripotency genes upon ESC differentiation

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2014-08-28 | E-GEOD-49305 | biostudies-arrayexpress

Calcineurin signaling regulates neural induction through antagonizing the BMP pathway

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2013-12-10 | E-GEOD-53106 | biostudies-arrayexpress

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AMPK governs lineage specification through Tfeb-dependent regulation of lysosomes

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Project description:Snai1 is a master factor of epithelial to mesenchymal transitioin (EMT), however, its role in embryonic stem cell (ESC) differentiation and lineage commitment remains undefined. We used microarrays to compare the global programme of gene expression between control and Snai1 knockout Flk1+ and Flk1- cells sorted from 4 day EBs. Control and Snai1 knockout ESCs were cultured as embryoid bodies in spotaneous differentiation media, 4 days EBs were dissociated and sorted by anti-Flk1 antibody to separated Flk1+ and Flk1- cells, total RNA were collected for Affymetrix microarrays

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