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Evaluation of covS polymorphisms on group A streptococcal global gene expression

ABSTRACT: We sought to determine how single amino acid changes in the active site of CovS autophosphorylation and subsequent phosphorylation of the response regulator CovR affect the gene expression profile of group A streptococcus There were 6 strains analyzed, each in quadruplicate replicates: 1) wild-type GAS; 2) covS deletion strain; 3) covS with alanine at AA 280; 4) covS with valine at AA 281; 5) covS with valine at AA 284; 6) covS with alanine at AA 457

ORGANISM(S): Streptococcus sp. 'group A'

SUBMITTER: Samuel Shelburne

PROVIDER: E-GEOD-65226 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of the effect of the histidine kinase CovS on response regulator phosphorylation in group A Streptococcus.

Horstmann Nicola N Sahasrabhojane Pranoti P Saldaña Miguel M Ajami Nadim J NJ Flores Anthony R AR Sumby Paul P Liu Chang-Gong CG Yao Hui H Su Xiaoping X Thompson Erika E Shelburne Samuel A SA

Infection and immunity 20150105 3

Two-component gene regulatory systems (TCSs) are a major mechanism by which bacteria respond to environmental stimuli and thus are critical to infectivity. For example, the control of virulence regulator/sensor kinase (CovRS) TCS is central to the virulence of the major human pathogen group A Streptococcus (GAS). Here, we used a combination of quantitative in vivo phosphorylation assays, isoallelic strains that varied by only a single amino acid in CovS, and transcriptome analyses to characteriz ...[more]

PMID: 25561708

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