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Identification of transcripts regulated by CEBPA protein and its P30 isoform in K562

ABSTRACT: To investigate the effect of CEBPA and its mutant isoform P30 on the expression of mRNAs and long non-coding RNAs (lncRNAs), we utilized the K562 AML cell line carrying a stable and Tet-on inducible CEBPA or P30 allele. Based on the expression of known CEBPA transcriptional targets, we selected RNA extracted from 48 hours of induction (CEBPA or P30) together with RNA extracted from control-induced cells (CTR). 2 biological replicates for each sample have been utilized.

ORGANISM(S): Homo sapiens

SUBMITTER: Alessandro Fatica

PROVIDER: E-GEOD-65235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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C/EBPα-p30 protein induces expression of the oncogenic long non-coding RNA UCA1 in acute myeloid leukemia.

Hughes James M JM Legnini Ivano I Salvatori Beatrice B Masciarelli Silvia S Marchioni Marcella M Fazi Francesco F Morlando Mariangela M Bozzoni Irene I Fatica Alessandro A

Oncotarget 20150701 21

Accumulating evidences indicate that different long non-coding RNAs (lncRNAs) might play a relevant role in tumorigenesis, with their expression and function already associated to cancer development and progression. CCAAT/enhancer-binding protein-α (CEBPA) is a critical regulator of myeloid differentiation whose inactivation contributes to the development of acute myeloid leukemia (AML). Mutations in C/EBPα occur in around 10% of AML cases, leading to the expression of a 30-kDa dominant negative ...[more]

PMID: 26053097

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