Unknown,Transcriptomics,Genomics,Proteomics

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Alternative RNA processing events in a double NSR mutant Arabidopsis


ABSTRACT: We analyze the effect of a double deletion mutant for alternative-splicing regulators nsra and nsrb (Nuclear Speckle RNA binding proteins), on the Arabidopsis thaliana transcriptome. RNA-seq experiments (polyA+ RNA) in triplicates for each condition WT and nsrab mutants.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: souiai oussema 

PROVIDER: E-GEOD-65717 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Detection of generic differential RNA processing events from RNA-seq data.

Tran Van Du T Vdu T   Souiai Oussema O   Romero-Barrios Natali N   Crespi Martin M   Gautheret Daniel D  

RNA biology 20160101 1


RNA-seq data analysis has revealed abundant alternative splicing in eukaryotic mRNAs. However, splicing is only one of many processing events that transcripts may undergo during their lifetime. We present here RNAprof (RNA profile analysis), a program for the detection of differential processing events from the comparison of RNA-seq experiments. RNAprof implements a specific gene-level normalization procedure and compares RNA-seq coverage profiles at nucleotide resolution to detect regions of si  ...[more]

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