Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

46 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile analysis of Staphylococcus aureus treated with nisin

ABSTRACT: Staphylococcus aureus (S. aureus) has already to be one of the most commonly identified bacteria that cause food poisoning. S. aureus colonization in humans can cause serious infections, toxinoses and life threatening diseases. The bacteriocin nisin has been extensively used as potential natural preservative in the food industry, but the overall transcriptional response mechanisms of S. aureus to nisin are still poorly understood. To detect the possible molecular mechanism of nisin against S. aureus, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus cells triggered by treatment with sub-inhibitory concentrations of nisin. Staphylococcus aureus planktonic cells were exposed for 60 minutes to nisin at concentration of 4 M-NM-

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Na Guo

PROVIDER: E-GEOD-65750 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Phenotype and expression profile analysis of Staphylococcus aureus biofilms and planktonic cells in response to Licochalcone A

Project description:Staphylococcus aureus (S. aureus) is one of the most important pathogens in humans and animals. The formation of S. aureus biofilm is considered an important mechanism of antimicrobial resistance. Therefore, finding effective drugs against the biofilm produced by S. aureus has been a high priority. Licochalcone A, a natural plant product, was reported to have antibacterial activities and showed good activity against all 21 tested strains of S. aureus biofilm and planktonic cells. To detect the possible molecular mechanism of Licochalcone A against S. aureus biofillm or planktonic cells, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus biofilm and planktonic cells triggered by treatment with sub-bactericidal and sub-inhibitory concentrations of Licochalcone A, respectively. Staphylococcus aureus planktonic cells and biofilm were exposed for 60 minutes to Licochalcone A at concentration of 2 M-NM-<g/ml (1/2M-CM-^W MIC) and 64 M-NM-<g/ml (4M-CM-^W MIBC), respectively. 4 samples including 4 control samples are analyzed.

2014-07-01 | E-GEOD-58938 | biostudies-arrayexpress

Gene expression profile analysis of Staphylococcus aureus treated with nisin

Project description:Staphylococcus aureus (S. aureus) has already to be one of the most commonly identified bacteria that cause food poisoning. S. aureus colonization in humans can cause serious infections, toxinoses and life threatening diseases. The bacteriocin nisin has been extensively used as potential natural preservative in the food industry, but the overall transcriptional response mechanisms of S. aureus to nisin are still poorly understood. To detect the possible molecular mechanism of nisin against S. aureus, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus cells triggered by treatment with sub-inhibitory concentrations of nisin.

2015-02-10 | GSE65750 | GEO

Sa MW2 vs SAN2

Project description:Transcriptional profiling of Staphylococcus aureus comparing MW2 strain with SAN2 strain which was nisin A high resistant strain.

2020-02-24 | GSE131352 | GEO

Phenotype and RNA-Seq-Based Transcriptome Profiling of Staphylococcus aureus biofilms in response to Tea tree oil

Project description:RNA sequencing (RNA-Seq) was used in our study to elucidate the mechanism of Tea tree oil (TTO) as a potential antibacterial agent to evaluate differentially expressed genes and functional network analysis in S. aureus ATCC 29213 biofilms. Staphylococcus aureus biofilm cells were exposed for 60 minutes to TTO at concentration of 1/2ÃMBIC (1 mg/ml).2 samples including 2 control samples are analyzed.

2016-08-19 | E-GEOD-85787 | biostudies-arrayexpress

Transcription profiling by array of Staphylococcus aureus grown in cheese matrix in mixed culture with Lactococcus lactis

Project description:Effect of the presence of Lactococcus lactis on Staphylococcus aureus transcriptome in cheese matrix. S. aureus was co-cultured with L. lactis LD61 in cheese matrix during 7 days. RNA samples were extracted at different time points (6 h, 8 h, 10 h, 24 h and 7 days) in order to monitor the dynamic response of S. aureus MW2 in cheese matrix in presence of L. lactis

2011-07-01 | E-MEXP-2842 | biostudies-arrayexpress

RNA-seq of Staphylococcus aureus human and bovine strains cultured in TSB and 50% milk media

Project description:The aim of this experiment was to understand the different transcriptomic profiles of Staphylococcus aureus strains when cultured in 50% milk media with TSB media as a control. Bovine S. aureus strains that are capable of clotting milk where compared to both bovine and human S. aureus strains unable to clot milk. All strains are from sequence type 97.

2024-09-04 | E-MTAB-14384 | biostudies-arrayexpress

Global transcriptional profiling of Staphylococcus aureus treated with eugenol

Project description:Staphylococcus aureus (S. aureus) is an important human and animal pathogen, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. eugenol, a natural plant product, has potent antimicrobial activity against S. aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with eugenol. Keywords: gene expression array-based, count Staphylococcus aureus cells were exposed for 45 minutes to eugenol at concentration of 250µg/ml (1/2× MIC), 2 samples including 1 control samples are analyzed.

2007-08-10 | E-GEOD-8735 | biostudies-arrayexpress

Extracellular biofilm matrix proteins in Staphylococcus aureus

Project description:Comparison of biofilm versus planktonic mode of growth in Staphylococcus aureus

2014-01-18 | E-MEXP-3924 | biostudies-arrayexpress

Transcription profiling by array of Staphylococcus aureus wild type and rnc, pnp and sigB mutants

Project description:Transcriptome mapping of Staphylococcus aureus wild type and rnc, pnp and sigB mutants

2010-12-31 | E-MEXP-2778 | biostudies-arrayexpress

Changes in the transcriptome of S. aureus SH1000 containing a nisin resistance mutation in nsaS

Project description:We have demonstrated previously that high-level resistance to nisin can occur in Staphylococcus aureus as a consequence of a single non-synonymous mutation in nsaS, which encodes a putative sensor kinase. To explore the mechanism by which this mutation confers high-level resistance we compared global transcriptomes of SH1000 and SH1000 (NsaS A208E) using RNAseq. This process identified several genes to be upregulated in SH1000 (NsaS A208E), including members of the NsaRS regulon which encode VraDE and BraDE, two putative ABC-transporters and are known to provide intrinsic nisin resistance. Gene deletion and complementation experiments revealed that both BraDE and VraDE are essential to high-level nisin resistance, with BraDE required for signal transduction through NsaRS, and VraDE directly responsible for nisin detoxification.

2018-11-19 | GSE114706 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data