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DNA methylation analysis by RRBS in germ cells of mice exposed to Vinclozolin

ABSTRACT: We provide quantitative maps of cytosine methylation at single base resolution by RRBS in E13.5 primordial germ cells and adult sperm purified from mice exposed to Vinclozolin. Pregnant F0 female mice were exposed to two doses (a low dose VD1=1 mg/kg bw/d and a high dose VD2=100mg/kg bw/d) of Vinclozolin in the drinking water during pregnancy. We isolated primordial germ cells at E13.5 and mature sperm from adult F1 males obtained from control and exposed mothers. For PGCs, we sequenced RRBS libraries prepared from one pool isolated from F1 embryos exposed to the low dose, one pool isolated from F1 embryos exposed to the high dose, and two control pools isolated from unexposed embryos. For sperm, we sequenced three RRBS libraries prepared from a pool of sperm isolated from F1 males exposed to the high dose, and five RRBS libraries prepared from control pools isolated from unexposed animals.

ORGANISM(S): Mus musculus

SUBMITTER: Michael Weber 

PROVIDER: E-GEOD-65784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells.

Brieño-Enríquez Miguel A MA   García-López Jesús J   Cárdenas David B DB   Guibert Sylvain S   Cleroux Elouan E   Děd Lukas L   Hourcade Juan de Dios Jde D   Pěknicová Jana J   Weber Michael M   Del Mazo Jesús J  

PloS one 20150421 4

In mammals, germ cell differentiation is initiated in the Primordial Germ Cells (PGCs) during fetal development. Prenatal exposure to environmental toxicants such as endocrine disruptors may alter PGC differentiation, development of the male germline and induce transgenerational epigenetic disorders. The anti-androgenic compound vinclozolin represents a paradigmatic example of molecule causing transgenerational effects on germ cells. We performed prenatal exposure to vinclozolin in mice and anal  ...[more]

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