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Gene expression analysis to characterize similarities and differences in T. canis- or T. cati-induced neurotoxocarosis

ABSTRACT: Larvae of Toxocara canis and T. cati show affinities to different tissues within the paratenic host. T. canis preferably migrates to the CNS whereat T. cati prefers muscle tissue. As both species share many characteristics like antigen fractions, reasons for this behaviour as well as underlying pathomechanisms or host reactions towards the respective parasite are unknown. Therefore, it was aimed to characterize the general pathogenesis as well as the respective host's reaction on the transcriptional level to identify similarities and differences between T. canis- and T. cati-infected brains. Transcriptional changes in cerebra as well as cerebella of T. canis- and T. cati-infected C57Bl/6J mice were analysed 42 days post infection. In each infection group, 3 animals were included and 4 animals in the uninfected control.

ORGANISM(S): Mus musculus

SUBMITTER: Robert Geffers

PROVIDER: E-GEOD-66094 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression analysis to characterize similarities and differences in T. canis- or T. cati-induced neurotoxocarosis

Project description:Larvae of Toxocara canis and T. cati show affinities to different tissues within the paratenic host. T. canis preferably migrates to the CNS whereat T. cati prefers muscle tissue. As both species share many characteristics like antigen fractions, reasons for this behaviour as well as underlying pathomechanisms or host reactions towards the respective parasite are unknown. Therefore, it was aimed to characterize the general pathogenesis as well as the respective host's reaction on the transcriptional level to identify similarities and differences between T. canis- and T. cati-infected brains.

2015-12-31 | GSE66094 | GEO

Toxocara canis- and Toxocara cati-induced neurotoxocarosis is associated with comprehensive brain transcriptomic alterations

Project description:Migration and persistence of Toxocara canis and T. cati larvae in brains of paratenic hosts, including humans, may induce the disease neurotoxocarosis (NT). Along with various clinical symptoms, neurodegenerative and neuropsychiatric disorders have been described as a consequence of the disease. As most knowledge on NT is derived from only a few published clinical cases, information regarding underlying pathomechanisms and host’s response to Toxocara remain scarce. Therefore, it was aimed to characterize the general pathogenesis as well as the respective host's reaction on the transcriptional level in brains of T. canis- and T. cati C57BL/6J mice as a model for paratenic hosts.

2021-12-05 | GSE190123 | GEO

Comparative transcriptomic analyses of male and female adult Toxocara canis.

Project description:Toxocariasis is an important, neglected zoonosis caused mainly by Toxocara canis. Although our knowledge of helminth molecular biology is improving through completed draft genome projects, there is limited detailed information on the molecular biology of Toxocara species. Here, transcriptomic sequencing of male and female adult T. canis and comparative analyses were conducted. For each sex, two-thirds (66-67%) of quality-filtered reads mapped to the gene set of T. canis, and at least five reads mapped to each of 16,196 (87.1%) of all 18,596 genes, and 321 genes were specifically transcribed in female and 1467 in male T. canis. Genes differentially transcribed between the two sexes were identified, enriched biological processes and pathways linked to these genes established, and molecules associated with reproduction and development predicted. In addition, small RNA pathways involved in reproduction were characterized, but there was no evidence for piwi RNA pathways in adult T. canis. The results of this transcriptomic study should provide a useful basis to support investigations of the reproductive biology of T. canis and related nematodes.

2016-11-30 | GSE75536 | GEO

MicroRNAs of Toxocara canis and their predicted functional roles.

Project description:To encourage biological and biotechnological investigations on Toxocara canis, an important neglected tropical parasite, high-throughput sequencing was carried out to generate the sRNAs resource of adult Toxocara canis. In total, 11,632,676 and 10,723,433 sRNAs reads were yielded from male and female libraries, with similar proportion of small nucleolar RNA, micro RNA and small nuclear RNA. No PIWI-associated RNA was found in T. canis. 1,985 of male and 2,062 of female known miRNAs were respectively obtained, as well as 99 and 71 novel miRNAs in both genders. Comparative analysis was carried out on expression level which generated the lists of differential-expressed miRNAs, including 854 of male-specific expressed and 932 of female-specific expressed MiRNAs, and on sequence level which showed expression-sequence coordination. Based on Gene Ontology annotation and KEGG pathway analysis, the conservation and functional diversification of these differentially expressed miRNAs were investigated focusing on reproduction and larval development. 53 miRNA seed family, such as Tc-miR-1648/6090/7412/7931 and Tc-miR-698/1892/6795/6963/6980/7044/7078, were predicted to be involved in reproduction and development processes. Moreover, it was found that seed family with interspecies conservation, like ACCCGUA/ACCCUGU/CCCUGAG/GAAAGAC/GAGAUCA, have wide distribution in larvae, secretion and host serum, strongly suggesting the potential roles of these miRNAs in host and parasite interactions. This is the first exploration of sRNAs in T. canis, which would boost systematic biological investigations and encourage novel drugs vaccines and diagnostic tests.

2016-05-11 | GSE68710 | GEO

DNA methylome of Canis lupus dingo

Project description:Using WGBS we investigated blood DNA methylation profiles of Canis lupus dingo and determined putative regulatory elements (unmethlated regions (UMRs) and lowly methylated regions (LMRs).

2020-08-27 | GSE119099 | GEO

DNA methylome of Canis lupus basenji

Project description:Using WGBS we investigated blood DNA methylation profiles of Canis lupus basenji and determined putative regulatory elements (unmethlated regions, UMRs, and lowly methylated regions, LMRs).

2021-02-22 | GSE159396 | GEO

Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection (part 4)

Project description:Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. Our analysis identified transcriptional differences in macrophage infection between B. melitensis and B. canis genes involved in iron transport. Increased transcription of the TonB, enterobactin, and ferric anguibactin transport systems were observed in B. canis but not B. melitensis during infection of macrophages. Therefore, iron appears as an important requirement during the first 24h of infection by B. canis but not for B. melitensis and provides strategies for controlling these pathogens. Comparison of total bacterial RNA from Brucella canis infected murine macrophages to broth grown bacteria

2012-12-01 | E-GEOD-34503 | biostudies-arrayexpress

Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection (part 3)

Project description:Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. Our analysis identified transcriptional differences in macrophage infection between B. melitensis and B. canis genes involved in iron transport. Increased transcription of the TonB, enterobactin, and ferric anguibactin transport systems were observed in B. canis but not B. melitensis during infection of macrophages. Therefore, iron appears as an important requirement during the first 24h of infection by B. canis but not for B. melitensis and provides strategies for controlling these pathogens. comparison of total bacterial RNA from Brucella canis infected murine macrophages and broth grown bacteria

2012-12-01 | E-GEOD-34501 | biostudies-arrayexpress

Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis During Macrophage Infection (part 2)

2012-12-01 | E-GEOD-34498 | biostudies-arrayexpress

RNA-seq analysis of stimulated canine macrophage by Brucella canis

Project description:A lot of attempts have been made to understand the immunopathological mechanisms of Brucella canis infection because of the importance of the disease in both public and clinical aspects. However, previous mechanisms are not still revealed. Therefore, in vitro models, which mimic to in vivo infection route using a canine epithelial cell, D17 cell, and a canine macrophage, DH82 cell, was used to solve the clues by analysis of transcriptomes in the cells. In this study, a co-culture model was constructed using the two cells, D17 and DH82 cell lines with trans-well plate. Also, a single cell culture system using DH82 was established. After stimulation of the cells in two different systems with B. canis, gene expressions in the macrophages of the two different system were analyzed by RNA-sequencing.

2019-07-18 | GSE134331 | GEO

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