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A study of the effect of OxLDL on C/EBP beta binding in human macrophages using chromatin immunoprecipitation for C/EBP beta with high throughput sequencing (ChIP-seq)

ABSTRACT: We report the C/EBP beta transcription factor binding landscape in human macrophages and oxLDL-induced foam cells ChIP-seq for C/EBP beta binding was performed in human macrophages and oxLDL induced-foam cells with quadruple biological replicates

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher O'Callaghan

PROVIDER: E-GEOD-66192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding.

Reschen Michael E ME Gaulton Kyle J KJ Lin Da D Soilleux Elizabeth J EJ Morris Andrew J AJ Smyth Susan S SS O'Callaghan Christopher A CA

PLoS genetics 20150402 4

Genome-wide association studies (GWAS) have identified over 40 loci that affect risk of coronary artery disease (CAD) and the causal mechanisms at the majority of loci are unknown. Recent studies have suggested that many causal GWAS variants influence disease through altered transcriptional regulation in disease-relevant cell types. We explored changes in transcriptional regulation during a key pathophysiological event in CAD, the environmental lipid-induced transformation of macrophages to lipi ...[more]

PMID: 25835000

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