Unknown,Transcriptomics,Genomics,Proteomics

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Differentiation of fiber initials on cotton ovules


ABSTRACT: Transcriptional profiling of cotton ovule cells. Comparison of expression of genes in cultured ovules (-1 DPA) treated with or without phytohormones (5um IAA and 1um GA) and over a time-course from 0 to 12 hours. The phytohormone incubation periods for unfertilized ovules were 0, 1, 3, 6, and 12 hours (H) with two biological replicates at each time-point.

ORGANISM(S): Gossypium hirsutum

SUBMITTER: Kathleen Yeater 

PROVIDER: E-GEOD-66251 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.

Kim Hee Jin HJ   Hinchliffe Doug J DJ   Triplett Barbara A BA   Chen Z Jeffrey ZJ   Stelly David M DM   Yeater Kathleen M KM   Moon Hong S HS   Gilbert Matthew K MK   Thyssen Gregory N GN   Turley Rickie B RB   Fang David D DD  

PloS one 20150430 4


The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for fiber initial differentiation have been discovered. To identify putative genes directly involved in the fiber initiation process, we used a cotton ovule culture technique that controls the timing of  ...[more]

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