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RNA-Seq of DRG and dorsal cord after nerve injury

ABSTRACT: Genes are up and down regualted in DRG and spinal dorsal cord after peripheral nerve injury WT male adult with sciatic and femoral nerve transection 7 days, RNA was purified from ipilateral or contralateral L4-L6 DRGs or lumbar spinal dorsal cords

ORGANISM(S): Mus musculus

SUBMITTER: Zhonghui Guan

PROVIDER: E-GEOD-66619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Injured sensory neuron-derived CSF1 induces microglial proliferation and DAP12-dependent pain.

Guan Zhonghui Z Kuhn Julia A JA Wang Xidao X Colquitt Bradley B Solorzano Carlos C Vaman Smitha S Guan Andrew K AK Evans-Reinsch Zoe Z Braz Joao J Devor Marshall M Abboud-Werner Sherry L SL Lanier Lewis L LL Lomvardas Stavros S Basbaum Allan I AI

Nature neuroscience 20151207 1

Although microglia have been implicated in nerve injury-induced neuropathic pain, the manner by which injured sensory neurons engage microglia remains unclear. We found that peripheral nerve injury induced de novo expression of colony-stimulating factor 1 (CSF1) in injured sensory neurons. CSF1 was transported to the spinal cord, where it targeted the microglial CSF1 receptor (CSF1R). Cre-mediated sensory neuron deletion of Csf1 completely prevented nerve injury-induced mechanical hypersensitivi ...[more]

PMID: 26642091

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