Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiles of human iPSC derived podocytes


ABSTRACT: The gene expression profile of human iPSC derived podocytes were analyzed by utilizing NEPHS1-GFP knock-in human iPS cell line. The induced podocytes shows characteristic gene expression pattern that overlap with those of mouse and human podocytes in vivo. Kidney tissue was induced from human iPSC by our originally established protocol. NEPHS1-GPF knock-in human iPSC was used to isolate podocytes from induced kidney tissues. To identify the molecules which are specifically expressed in induced podocytes, NEPHS1-GFP positive and negative fractions were sorted by FACS and compared. RNA was isolated from cells and the gene expression profiles were determined by microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Atsuhiro Taguchi 

PROVIDER: E-GEOD-66969 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation.

Sharmin Sazia S   Taguchi Atsuhiro A   Kaku Yusuke Y   Yoshimura Yasuhiro Y   Ohmori Tomoko T   Sakuma Tetsushi T   Mukoyama Masashi M   Yamamoto Takashi T   Kurihara Hidetake H   Nishinakamura Ryuichi R  

Journal of the American Society of Nephrology : JASN 20151119 6


Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator-like effector n  ...[more]

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