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Contribution of WDR77 and p53 to androgen response in prostate cancer cells

ABSTRACT: To decipher the contribution of WDR77 and p53 to androgen-responsive gene expression, effect of siRNA-mediated silencing of WDR77 and p53 on expression of androgen-dependent genes was studied. Human LNCaP prostate cancer cells were transfected with individual siRNA SmartPools targeting WDR77 or p53 or a non-targeting siRNA SmartPool. Forty-two hours after transfection, cells were treated with synthetic androgen R1881 (5nM) or vehicle. Three biological replicates were generated per treatment group. Forty-eight hours later, total RNA was isolated and processed for Illumina oligoarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Qiang Hu

PROVIDER: E-GEOD-66977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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