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RNAi of TbNMD3 in procyclic form Trypanosoma brucei lines

ABSTRACT: A procyclic form Trypansome brucei RNAi line (PTT parental line, transfected with pALC14 incorporating a TbNMD3 gene fragment) capable of inducing depletion of TbNMD3 was analysed for mRNA expression by RNAseq Cells were grown for 72 hours in culture; RNAi was induced in cells by the addition of 1 microgram/ml of tetracycline

ORGANISM(S): Trypanosoma brucei brucei

SUBMITTER: Alasdair Ivens

PROVIDER: E-GEOD-67246 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway.

Bühlmann Melanie M Walrad Pegine P Rico Eva E Ivens Alasdair A Capewell Paul P Naguleswaran Arunasalam A Roditi Isabel I Matthews Keith R KR

Nucleic acids research 20150414 9

Trypanosomes mostly regulate gene expression through post-transcriptional mechanisms, particularly mRNA stability. However, much mRNA degradation is cytoplasmic such that mRNA nuclear export must represent an important level of regulation. Ribosomal RNAs must also be exported from the nucleus and the trypanosome orthologue of NMD3 has been confirmed to be involved in rRNA processing and export, matching its function in other organisms. Surprisingly, we found that TbNMD3 depletion also generates ...[more]

PMID: 25873624

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