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MicroRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO4]

ABSTRACT: Analysis of gene expression in ovarian cancer cells with and without overexpression of the miRNA mir-124 which will provide data about genes possibly directly regulated by miR-124. Also, analysis of gene expression in ovarian cancer cells with and without knockdown of the homeodomain transcription factor SIX4 (a target of miR-124) which will provide data about genes possibly directly regulated by SIX4 Total RNA was isolated from PEO4 cells transfected with miRNA mimic negative control (miNC), miR-124 mimic, siRNA negative control (siNC) oligo, or siRNA targeting the SIX4 gene (siSIX4) 72 hours post-transfection

ORGANISM(S): Homo sapiens

SUBMITTER: Michael White

PROVIDER: E-GEOD-67328 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO1]

Project description:Analysis of gene expression in ovarian cancer cells with and without overexpression of the miRNA mir-124 which will provide data about genes possibly directly regulated by miR-124. Also, analysis of gene expression in ovarian cancer cells with and without knockdown of the homeodomain transcription factor SIX4 (a target of miR-124) which will provide data about genes possibly directly regulated by SIX4 Total RNA was isolated from PEO1 cells transfected with miRNA mimic negative control (miNC), miR-124 mimic, siRNA negative control (siNC) oligo, or siRNA targeting the SIX4 gene (siSIX4) 72 hours post-transfection

2015-03-27 | E-GEOD-67327 | biostudies-arrayexpress

microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO4]

2015-03-27 | GSE67328 | GEO

microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells [PEO1]

2015-03-27 | GSE67327 | GEO

Expression profiling of ovarian cancer cell lines

Project description:Paired platinum-sensitive & platinum-resistant cell lines PEO1 and PEO4 (respectively) were derived from the same patient. Cell line cultures were profiled with the HT-HGU133a GeneChip to investigate chemotherapy resistance related expression of genes marked with different histone modifications in a primary ovarian tumour (clinical data for the primary ovarian tumour is available as Series supplementary file). 4 replicates of each of the PEO1 and PEO4 cell line were profiled using the HT-HGU133a GeneChip

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microRNA Induction of Fatal Differentiation Programs is a Context-selective Vulnerability in Ovarian Cancer Cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-03-27 | E-GEOD-67330 | biostudies-arrayexpress

miR-124 coordinates metabolic regulators acting at early stages of human neurogenesis

Project description:Metabolic dysregulation of neurons is associated with diverse human brain disorders. Metabolic reprogramming occurs during neuronal differentiation, but it is not fully understood which molecules regulate metabolic changes at the early stages of neurogenesis. In this study, we report that miR-124 is a driver of metabolic change at the initiating stage of human neurogenesis. Proteome analysis has shown the oxidative phosphorylation pathway to be the most significantly altered among the differentially expressed proteins (DEPs) in the immature neurons after the knockdown of miR-124. In agreement with these proteomics results, miR-124-depleted neurons display mitochondrial dysfunctions, such as decreased mitochondrial membrane potential and cellular respiration. Moreover, morphological analyses of mitochondria in early differentiated neurons after miR-124 knockdown result in smaller and less mature shapes. Lastly, we show the potential of identified DEPs as novel metabolic regulators in early neuronal development by validating the effects of GSTK1 on cellular respiration. GSTK1, which is upregulated most significantly in miR-124 knockdown neurons, reduces the oxygen consumption rate of neural cells. Collectively, our data highlight the roles of miR-124 in coordinating metabolic maturation at the early stages of neurogenesis and provide insights into potential metabolic regulators associated with human brain disorders characterised by metabolic dysfunctions.

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Network-based integration of multi-omics data identifies the determinants of miR-491-5p effects

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Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12

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Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12

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2010-07-30 | E-GEOD-19967 | biostudies-arrayexpress