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Analysis of gut microbiota by pyrosequencing of 16S rRNA gene in F344 rats


ABSTRACT: Chronic acid suppression by proton pump inhibitor (PPI) has been hypothesized to alter the gut microbiota via a change in intestinal pH. To evaluate the changes in gut microbiota composition by long-term PPI treatment. Twenty-four week old F344 rats were fed with (n = 5) or without (n = 6) lansoprazole (PPI) for 50 weeks. Then, profiles of luminal microbiota in the terminal ileum were analyzed. Pyrosequencing for 16S rRNA gene was performed by genome sequencer FLX (454 Life Sciences/Roche) and analyzed by metagenomic bioinformatics.

ORGANISM(S): rat gut metagenome

SUBMITTER: Nayoung Kim 

PROVIDER: E-GEOD-67800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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