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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HeLa cells stimulated with epidermal growth factor vs. unstimulated sampled at multiple time points to investigate the temporal regulation of the cellular EGFR cascade

ABSTRACT: HELA cells derived from human cervical tumor were subjected to EGF stimulation for 0,20,40,60,120,240 and 480 minutes. We used microarrays to understand the temporal regulation of the cellular EGFR cascade. Experiment Overall Design: HELA cells were grown in DMEM supplemented with 10% FCS and antibiotics. Cells were serum deprived for 24 hours. Following stimulation with 10 ng/ml EGF for 0,20,40,60,120,240 and 480 minutes.

ORGANISM(S): Homo sapiens

SUBMITTER: Tal Shay

PROVIDER: E-GEOD-6783 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A module of negative feedback regulators defines growth factor signaling.

Amit Ido I Citri Ami A Shay Tal T Lu Yiling Y Katz Menachem M Zhang Fan F Tarcic Gabi G Siwak Doris D Lahad John J Jacob-Hirsch Jasmine J Amariglio Ninette N Vaisman Nora N Segal Eran E Rechavi Gideon G Alon Uri U Mills Gordon B GB Domany Eytan E Yarden Yosef Y

Nature genetics 20070225 4

Signaling pathways invoke interplays between forward signaling and feedback to drive robust cellular response. In this study, we address the dynamics of growth factor signaling through profiling of protein phosphorylation and gene expression, demonstrating the presence of a kinetically defined cluster of delayed early genes that function to attenuate the early events of growth factor signaling. Using epidermal growth factor receptor signaling as the major model system and concentrating on regula ...[more]

PMID: 17322878

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