Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Peter 't Hoen
PROVIDER: E-GEOD-6790 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
't Hoen Peter A C PA de Meijer Emile J EJ Boer Judith M JM Vossen Rolf H A M RH Turk Rolf R Maatman Ronald G H J RG Davies Kay E KE van Ommen Gert-Jan B GJ van Deutekom Judith C T JC den Dunnen Johan T JT
The Journal of biological chemistry 20071213 9
We report the generation of mice with an intact and functional copy of the 2.3-megabase human dystrophin gene (hDMD), the largest functional stretch of human DNA thus far integrated into a mouse chromosome. Yeast spheroplasts containing an artificial chromosome with the full-length hDMD gene were fused with mouse embryonic stem cells and were subsequently injected into mouse blastocysts to produce transgenic hDMD mice. Human-specific PCR, Southern blotting, and fluorescent in situ hybridization ...[more]