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A novel bivalent chromatin state exists at enhancers

ABSTRACT: We assess global chromatin accessibility following 5-Aza-CdR treatment of HCT116 cells Simultaneous genome-wide mapping of DNA methylation and nucleosome occupancy of HCT116 cells

ORGANISM(S): Homo sapiens

SUBMITTER: Jessica Charlet

PROVIDER: E-GEOD-68046 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bivalent Regions of Cytosine Methylation and H3K27 Acetylation Suggest an Active Role for DNA Methylation at Enhancers.

Charlet Jessica J Duymich Christopher E CE Lay Fides D FD Mundbjerg Kamilla K Dalsgaard Sørensen Karina K Liang Gangning G Jones Peter A PA

Molecular cell 20160501 3

The role of cytosine methylation in the structure and function of enhancers is not well understood. In this study, we investigate the role of DNA methylation at enhancers by comparing the epigenomes of the HCT116 cell line and its highly demethylated derivative, DKO1. Unlike promoters, a portion of regular and super- or stretch enhancers show active H3K27ac marks co-existing with extensive DNA methylation, demonstrating the unexpected presence of bivalent chromatin in both cultured and unculture ...[more]

PMID: 27153539

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The effects of the global loss of DNA methylation on the functional cancer epigenome (Bisulfite-seq)

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